The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology

Nyles W. Charon, Stuart F. Goldstein, Michael Marko, Chyongere Hsieh, Linda L. Gebhardt, M. Abdul Motaleb, Charles William Wolgemuth, Ronald J. Limberger, Nancy Rowe

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cryotomography, we noted that membrane blebs formed at the ends of the cells. These blebs may be precursors to vesicles that are released from cells grown in vivo and in vitro. We found that the periplasmic space of B. burgdorferi was quite narrow (16.0 nm) compared to those of Escherichia coli and Pseudomonas aeruginosa. However, in the vicinity of the periplasmic flagella, this space was considerably wider (42.3 nm). In contrast to previous results, the periplasmic flagella did not form a bundle but rather formed a tight-fitting ribbon that wraps around the protoplasmic cell cylinder in a right-handed sense. We show how the ribbon configuration of the assembled periplasmic flagella is more advantageous than a bundle for both swimming and forming the flat-wave morphology. Previous results indicate that B. burgdorferi motility is dependent on the rotation of the periplasmic flagella in generating backward-moving waves along the length of the cell. This swimming requires that the rotation of the flagella exerts force on the cell cylinder. Accordingly, a ribbon is more beneficial than a bundle, as this configuration allows each periplasmic flagellum to have direct contact with the cell cylinder in order to exert that force, and it minimizes interference between the rotating filaments.

Original languageEnglish (US)
Pages (from-to)600-607
Number of pages8
JournalJournal of Bacteriology
Volume191
Issue number2
DOIs
StatePublished - Jan 2009
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Flagella
Periplasm
Blister
Electrons
Dehydration
Pseudomonas aeruginosa
Electron Microscopy
Staining and Labeling
Escherichia coli
Bacteria
Membranes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Charon, N. W., Goldstein, S. F., Marko, M., Hsieh, C., Gebhardt, L. L., Motaleb, M. A., ... Rowe, N. (2009). The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology. Journal of Bacteriology, 191(2), 600-607. https://doi.org/10.1128/JB.01288-08

The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology. / Charon, Nyles W.; Goldstein, Stuart F.; Marko, Michael; Hsieh, Chyongere; Gebhardt, Linda L.; Motaleb, M. Abdul; Wolgemuth, Charles William; Limberger, Ronald J.; Rowe, Nancy.

In: Journal of Bacteriology, Vol. 191, No. 2, 01.2009, p. 600-607.

Research output: Contribution to journalArticle

Charon, NW, Goldstein, SF, Marko, M, Hsieh, C, Gebhardt, LL, Motaleb, MA, Wolgemuth, CW, Limberger, RJ & Rowe, N 2009, 'The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology', Journal of Bacteriology, vol. 191, no. 2, pp. 600-607. https://doi.org/10.1128/JB.01288-08
Charon, Nyles W. ; Goldstein, Stuart F. ; Marko, Michael ; Hsieh, Chyongere ; Gebhardt, Linda L. ; Motaleb, M. Abdul ; Wolgemuth, Charles William ; Limberger, Ronald J. ; Rowe, Nancy. / The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology. In: Journal of Bacteriology. 2009 ; Vol. 191, No. 2. pp. 600-607.
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