The frequency of 1,4-Benzoquinone-lysine adducts in cytochrome c correlate with defects in apoptosome activation

Ashley A. Fisher, Matthew T. Labenski, Srinivas Malladi, John D. Chapman, Shawn B. Bratton, Terrence Monks, Serrine Lau

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Electrophile-mediated post-translational modifications (PTMs) are known to cause tissue toxicities and disease progression. These effects are mediated via site-specific modifications and structural disruptions associated with such modifications. 1,4-Benzoquinone (BQ) and its quinone-thioether metabolites are electrophiles that elicit their toxicity via protein arylation and the generation of reactive oxygen species. Site-specific BQ-lysine adducts are found on residues in cytochrome c that are necessary for protein-protein interactions, and these adducts contribute to interferences in its ability to facilitate apoptosome formation. To further characterize the structural and functional impact of these BQ-mediated PTMs, the original mixture of BQ-adducted cytochrome c was fractionated by liquid isoelectric focusing to provide various fractions of BQadducted cytochrome c species devoid of the native protein. The fractionation process separates samples based on their isoelectric point (pI), and because BQ adducts form predominantly on lysine residues, increased numbers of BQ adducts on cytochrome c correlate with a lower protein pI. Each fraction was analyzed for structural changes, and each was also assayed for the ability to support apoptosome-mediated activation of caspase-3. Circular dichroism revealed that several of the BQ-adducted cytochrome c species maintained a slightly more rigid structure in comparison to native cytochrome c. BQ-adducted cytochrome c also failed to activate caspase-3, with increasing numbers of BQ-lysine adducts corresponding to a greater inability to activate the apoptosome. In summary, the specific site of the BQ-lysine adducts, and the nature of the adduct, are important determinants of the subsequent structural changes to cytochrome c. In particular, adducts at sites necessary for protein-protein interactions interfere with the proapoptotic function of cytochrome c.

Original languageEnglish (US)
Pages (from-to)64-72
Number of pages9
JournalToxicological Sciences
Volume122
Issue number1
DOIs
StatePublished - 2011

Fingerprint

Apoptosomes
Cytochromes c
Lysine
Chemical activation
Defects
Proteins
Post Translational Protein Processing
Caspase 3
Toxicity
benzoquinone
Rigid structures
Isoelectric Point
Isoelectric Focusing
Sulfides
Fractionation
Metabolites
Circular Dichroism
Disease Progression

Keywords

  • 1,4-benzoquinone
  • Apoptosome formation
  • Cytochrome c
  • Fractionation
  • Liquid IEF separation
  • Posttranslational modification

ASJC Scopus subject areas

  • Toxicology

Cite this

The frequency of 1,4-Benzoquinone-lysine adducts in cytochrome c correlate with defects in apoptosome activation. / Fisher, Ashley A.; Labenski, Matthew T.; Malladi, Srinivas; Chapman, John D.; Bratton, Shawn B.; Monks, Terrence; Lau, Serrine.

In: Toxicological Sciences, Vol. 122, No. 1, 2011, p. 64-72.

Research output: Contribution to journalArticle

Fisher, Ashley A. ; Labenski, Matthew T. ; Malladi, Srinivas ; Chapman, John D. ; Bratton, Shawn B. ; Monks, Terrence ; Lau, Serrine. / The frequency of 1,4-Benzoquinone-lysine adducts in cytochrome c correlate with defects in apoptosome activation. In: Toxicological Sciences. 2011 ; Vol. 122, No. 1. pp. 64-72.
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AU - Fisher, Ashley A.

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AB - Electrophile-mediated post-translational modifications (PTMs) are known to cause tissue toxicities and disease progression. These effects are mediated via site-specific modifications and structural disruptions associated with such modifications. 1,4-Benzoquinone (BQ) and its quinone-thioether metabolites are electrophiles that elicit their toxicity via protein arylation and the generation of reactive oxygen species. Site-specific BQ-lysine adducts are found on residues in cytochrome c that are necessary for protein-protein interactions, and these adducts contribute to interferences in its ability to facilitate apoptosome formation. To further characterize the structural and functional impact of these BQ-mediated PTMs, the original mixture of BQ-adducted cytochrome c was fractionated by liquid isoelectric focusing to provide various fractions of BQadducted cytochrome c species devoid of the native protein. The fractionation process separates samples based on their isoelectric point (pI), and because BQ adducts form predominantly on lysine residues, increased numbers of BQ adducts on cytochrome c correlate with a lower protein pI. Each fraction was analyzed for structural changes, and each was also assayed for the ability to support apoptosome-mediated activation of caspase-3. Circular dichroism revealed that several of the BQ-adducted cytochrome c species maintained a slightly more rigid structure in comparison to native cytochrome c. BQ-adducted cytochrome c also failed to activate caspase-3, with increasing numbers of BQ-lysine adducts corresponding to a greater inability to activate the apoptosome. In summary, the specific site of the BQ-lysine adducts, and the nature of the adduct, are important determinants of the subsequent structural changes to cytochrome c. In particular, adducts at sites necessary for protein-protein interactions interfere with the proapoptotic function of cytochrome c.

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