The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen

Aikseng Ooi, Sianghee Tan, Rosmawati Mohamed, Noorsaadah Abdul Rahman, Rofina Yasmin Othman

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.

Original languageEnglish (US)
Pages (from-to)471-481
Number of pages11
JournalJournal of Biotechnology
Volume121
Issue number4
DOIs
StatePublished - Feb 24 2006
Externally publishedYes

Keywords

  • CGMMV
  • Chimeric virus
  • HBsAg
  • Infectious transcripts
  • Tobamovirus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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