The generation of a colE1-Apr cloning vehicle which allows detection of inserted DNA

Magdalene So, Ron Gill, Stanley Falkow

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A 3.2 Mdal sequence of DNA, TnA, which contains the ampicillin (Ap) resistance determinant has been translocated from an R plasmid to the plasmid ColE1. A total of 12 isolates were studied. There are at least 8 sites in ColE1 at which TnA has inserted. Insertion at five of these has resulted in a Col- phenotype. One ColE1-Apr plasmid, RSF2124, was examined further and its replication properties are found to be similar to that of the parent plasmid. RSF2124 appears to be a useful plasmid vehicle for the molecular cloning of DNA from diverse prokaryotic sources: it codes for readily detectable Ap resistance and contains a single EcoRI site in a gene affecting colicin biosynthesis so that it is unable to produce colicin upon ligation to other DNA.

Original languageEnglish (US)
Pages (from-to)239-249
Number of pages11
JournalMGG Molecular & General Genetics
Issue number3
StatePublished - Sep 1975
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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