The genome sequence of the filamentous fungus Neurospora crassa

James E. Galagan, Sarah E. Calvo, Katherine A. Borkovich, Eric U. Selker, Nick O. Read, David Jaffe, William FitzHugh, Li Jun Ma, Serge Smirnov, Seth Purcell, Bushra Rehman, Timothy Elkins, Reinhard Engels, Shunguang Wang, Cydney B. Nielsen, Jonathan Butler, Matthew Endrizzi, Dayong Qui, Peter Ianakiev, Deborah Bell-PedersenMary Anne Nelson, Margaret Werner-Washburne, Claude P. Selitrennikoff, John A. Kinsey, Edward L. Braun, Alex Zelter, Ulrich Schulte, Gregory O. Kothe, Gregory Jedd, Werner Mewes, Chuck Staben, Edward Marcotte, David Greenberg, Alice Roy, Karen Foley, Jerome Naylor, Nicole Stange-Thomann, Robert Barrett, Sante Gnerre, Michael Kamal, Manolis Kamvysselis, Evan Mauceli, Cord Bielke, Stephen Rudd, Dmitrij Frishman, Svetlana Krystofova, Carolyn Rasmussen, Robert L. Metzenberg, David D. Perkins, Scott Kroken, Carlo Cogoni, Giuseppe Macino, David Catcheside, Weixi Li, Robert J. Pratt, Stephen A. Osmani, Colin P C DeSouza, Louise Glass, Marc Joel Orbach, J. Andrew Berglund, Rodger Voelker, Oded Yarden, Michael Plamann, Stephan Seiler, Jay Dunlap, Alan Radford, Rodolfo Aramayo, Donald O. Natvig, Lisa A. Alex, Gertrud Mannhaupt, Daniel J. Ebbole, Michael Freitag, Ian Paulsen, Matthew S. Sachs, Eric S. Lander, Chad Nusbaum, Bruce Birren

Research output: Contribution to journalArticle

1091 Citations (Scopus)

Abstract

Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report a high-quality draft sequence of the N. crassa genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes-more than twice as many as in the fission yeast Schizosaccharomyces pombe and only about 25% fewer than in the fruiffly Drosophila melanogaster. Analysis of the gene set yields insights into unexpected aspects of Neurospora biology including the identification of genes potentially associated with red light photobiology, genes implicated in secondary metabolism, and important differences in Ca2+ signalling as compared with plants and animals. Neurospora possesses the widest array of genome defence mechanisms known for any eukaryotic organism, including a process unique to fungi called repeat-induced point mutation (RIP). Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes.

Original languageEnglish (US)
Pages (from-to)859-868
Number of pages10
JournalNature
Volume422
Issue number6934
DOIs
StatePublished - Apr 24 2003

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Neurospora crassa
Fungi
Genome
Neurospora
Schizosaccharomyces
Genes
Point Mutation
20th Century History
Photobiology
Secondary Metabolism
Drosophila melanogaster
Biochemistry
Molecular Biology
Light
Proteins

ASJC Scopus subject areas

  • General

Cite this

Galagan, J. E., Calvo, S. E., Borkovich, K. A., Selker, E. U., Read, N. O., Jaffe, D., ... Birren, B. (2003). The genome sequence of the filamentous fungus Neurospora crassa. Nature, 422(6934), 859-868. https://doi.org/10.1038/nature01554

The genome sequence of the filamentous fungus Neurospora crassa. / Galagan, James E.; Calvo, Sarah E.; Borkovich, Katherine A.; Selker, Eric U.; Read, Nick O.; Jaffe, David; FitzHugh, William; Ma, Li Jun; Smirnov, Serge; Purcell, Seth; Rehman, Bushra; Elkins, Timothy; Engels, Reinhard; Wang, Shunguang; Nielsen, Cydney B.; Butler, Jonathan; Endrizzi, Matthew; Qui, Dayong; Ianakiev, Peter; Bell-Pedersen, Deborah; Nelson, Mary Anne; Werner-Washburne, Margaret; Selitrennikoff, Claude P.; Kinsey, John A.; Braun, Edward L.; Zelter, Alex; Schulte, Ulrich; Kothe, Gregory O.; Jedd, Gregory; Mewes, Werner; Staben, Chuck; Marcotte, Edward; Greenberg, David; Roy, Alice; Foley, Karen; Naylor, Jerome; Stange-Thomann, Nicole; Barrett, Robert; Gnerre, Sante; Kamal, Michael; Kamvysselis, Manolis; Mauceli, Evan; Bielke, Cord; Rudd, Stephen; Frishman, Dmitrij; Krystofova, Svetlana; Rasmussen, Carolyn; Metzenberg, Robert L.; Perkins, David D.; Kroken, Scott; Cogoni, Carlo; Macino, Giuseppe; Catcheside, David; Li, Weixi; Pratt, Robert J.; Osmani, Stephen A.; DeSouza, Colin P C; Glass, Louise; Orbach, Marc Joel; Berglund, J. Andrew; Voelker, Rodger; Yarden, Oded; Plamann, Michael; Seiler, Stephan; Dunlap, Jay; Radford, Alan; Aramayo, Rodolfo; Natvig, Donald O.; Alex, Lisa A.; Mannhaupt, Gertrud; Ebbole, Daniel J.; Freitag, Michael; Paulsen, Ian; Sachs, Matthew S.; Lander, Eric S.; Nusbaum, Chad; Birren, Bruce.

In: Nature, Vol. 422, No. 6934, 24.04.2003, p. 859-868.

Research output: Contribution to journalArticle

Galagan, JE, Calvo, SE, Borkovich, KA, Selker, EU, Read, NO, Jaffe, D, FitzHugh, W, Ma, LJ, Smirnov, S, Purcell, S, Rehman, B, Elkins, T, Engels, R, Wang, S, Nielsen, CB, Butler, J, Endrizzi, M, Qui, D, Ianakiev, P, Bell-Pedersen, D, Nelson, MA, Werner-Washburne, M, Selitrennikoff, CP, Kinsey, JA, Braun, EL, Zelter, A, Schulte, U, Kothe, GO, Jedd, G, Mewes, W, Staben, C, Marcotte, E, Greenberg, D, Roy, A, Foley, K, Naylor, J, Stange-Thomann, N, Barrett, R, Gnerre, S, Kamal, M, Kamvysselis, M, Mauceli, E, Bielke, C, Rudd, S, Frishman, D, Krystofova, S, Rasmussen, C, Metzenberg, RL, Perkins, DD, Kroken, S, Cogoni, C, Macino, G, Catcheside, D, Li, W, Pratt, RJ, Osmani, SA, DeSouza, CPC, Glass, L, Orbach, MJ, Berglund, JA, Voelker, R, Yarden, O, Plamann, M, Seiler, S, Dunlap, J, Radford, A, Aramayo, R, Natvig, DO, Alex, LA, Mannhaupt, G, Ebbole, DJ, Freitag, M, Paulsen, I, Sachs, MS, Lander, ES, Nusbaum, C & Birren, B 2003, 'The genome sequence of the filamentous fungus Neurospora crassa', Nature, vol. 422, no. 6934, pp. 859-868. https://doi.org/10.1038/nature01554
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read NO, Jaffe D et al. The genome sequence of the filamentous fungus Neurospora crassa. Nature. 2003 Apr 24;422(6934):859-868. https://doi.org/10.1038/nature01554
Galagan, James E. ; Calvo, Sarah E. ; Borkovich, Katherine A. ; Selker, Eric U. ; Read, Nick O. ; Jaffe, David ; FitzHugh, William ; Ma, Li Jun ; Smirnov, Serge ; Purcell, Seth ; Rehman, Bushra ; Elkins, Timothy ; Engels, Reinhard ; Wang, Shunguang ; Nielsen, Cydney B. ; Butler, Jonathan ; Endrizzi, Matthew ; Qui, Dayong ; Ianakiev, Peter ; Bell-Pedersen, Deborah ; Nelson, Mary Anne ; Werner-Washburne, Margaret ; Selitrennikoff, Claude P. ; Kinsey, John A. ; Braun, Edward L. ; Zelter, Alex ; Schulte, Ulrich ; Kothe, Gregory O. ; Jedd, Gregory ; Mewes, Werner ; Staben, Chuck ; Marcotte, Edward ; Greenberg, David ; Roy, Alice ; Foley, Karen ; Naylor, Jerome ; Stange-Thomann, Nicole ; Barrett, Robert ; Gnerre, Sante ; Kamal, Michael ; Kamvysselis, Manolis ; Mauceli, Evan ; Bielke, Cord ; Rudd, Stephen ; Frishman, Dmitrij ; Krystofova, Svetlana ; Rasmussen, Carolyn ; Metzenberg, Robert L. ; Perkins, David D. ; Kroken, Scott ; Cogoni, Carlo ; Macino, Giuseppe ; Catcheside, David ; Li, Weixi ; Pratt, Robert J. ; Osmani, Stephen A. ; DeSouza, Colin P C ; Glass, Louise ; Orbach, Marc Joel ; Berglund, J. Andrew ; Voelker, Rodger ; Yarden, Oded ; Plamann, Michael ; Seiler, Stephan ; Dunlap, Jay ; Radford, Alan ; Aramayo, Rodolfo ; Natvig, Donald O. ; Alex, Lisa A. ; Mannhaupt, Gertrud ; Ebbole, Daniel J. ; Freitag, Michael ; Paulsen, Ian ; Sachs, Matthew S. ; Lander, Eric S. ; Nusbaum, Chad ; Birren, Bruce. / The genome sequence of the filamentous fungus Neurospora crassa. In: Nature. 2003 ; Vol. 422, No. 6934. pp. 859-868.
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