The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis

William G. Rerie, Kenneth A Feldmann, M. David Marks

Research output: Contribution to journalArticle

389 Citations (Scopus)

Abstract

The GLABRA2 gene (GL2) is one of several genes known to have a role in trichome development in Arabidopsis. Mutations at this locus result in abnormal trichome expansion. We have identified several gl2 mutants from a T- DNA-mutagenized population of plants. The T-DNA insert in one of the mutant lines cosegregated with the recessive gl2 phenotype and thus served as a molecular tag to isolate genomic DNA at the putative GL2 locus. RFLP analysis of the segregating population and subsequent molecular complementation experiments established that the GL2 gene had been cloned. The predicted polypeptide from one of the ORFs contained on this fragment showed significant identity to the homeo domain sequence. The construction of a full-length cDNA by RT-PCR confirmed the presence of a homeo box in the GL2 gene and showed that it is substantially different from other recently cloned homeo box genes in plants. The expression pattern of GL2, as demonstrated by in situ hybridization, indicated that the gene is expressed in trichome progenitor cells and at stages associated with trichome development. This suggests that GL2 may regulate events required for the directional cell expansion observed during trichome formation.

Original languageEnglish (US)
Pages (from-to)1388-1399
Number of pages12
JournalGenes and Development
Volume8
Issue number12
StatePublished - Jun 15 1994

Fingerprint

Trichomes
Homeodomain Proteins
Arabidopsis
Genes
Homeobox Genes
Restriction Fragment Length Polymorphisms
Population
Open Reading Frames
In Situ Hybridization
Stem Cells
Complementary DNA

Keywords

  • Arabidopsis
  • GLABRA2 gene
  • homeo box
  • homeo domain protein
  • trichome development

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis. / Rerie, William G.; Feldmann, Kenneth A; Marks, M. David.

In: Genes and Development, Vol. 8, No. 12, 15.06.1994, p. 1388-1399.

Research output: Contribution to journalArticle

@article{d6b25678078c47df98b341c7dea13e4f,
title = "The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis",
abstract = "The GLABRA2 gene (GL2) is one of several genes known to have a role in trichome development in Arabidopsis. Mutations at this locus result in abnormal trichome expansion. We have identified several gl2 mutants from a T- DNA-mutagenized population of plants. The T-DNA insert in one of the mutant lines cosegregated with the recessive gl2 phenotype and thus served as a molecular tag to isolate genomic DNA at the putative GL2 locus. RFLP analysis of the segregating population and subsequent molecular complementation experiments established that the GL2 gene had been cloned. The predicted polypeptide from one of the ORFs contained on this fragment showed significant identity to the homeo domain sequence. The construction of a full-length cDNA by RT-PCR confirmed the presence of a homeo box in the GL2 gene and showed that it is substantially different from other recently cloned homeo box genes in plants. The expression pattern of GL2, as demonstrated by in situ hybridization, indicated that the gene is expressed in trichome progenitor cells and at stages associated with trichome development. This suggests that GL2 may regulate events required for the directional cell expansion observed during trichome formation.",
keywords = "Arabidopsis, GLABRA2 gene, homeo box, homeo domain protein, trichome development",
author = "Rerie, {William G.} and Feldmann, {Kenneth A} and Marks, {M. David}",
year = "1994",
month = "6",
day = "15",
language = "English (US)",
volume = "8",
pages = "1388--1399",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "12",

}

TY - JOUR

T1 - The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis

AU - Rerie, William G.

AU - Feldmann, Kenneth A

AU - Marks, M. David

PY - 1994/6/15

Y1 - 1994/6/15

N2 - The GLABRA2 gene (GL2) is one of several genes known to have a role in trichome development in Arabidopsis. Mutations at this locus result in abnormal trichome expansion. We have identified several gl2 mutants from a T- DNA-mutagenized population of plants. The T-DNA insert in one of the mutant lines cosegregated with the recessive gl2 phenotype and thus served as a molecular tag to isolate genomic DNA at the putative GL2 locus. RFLP analysis of the segregating population and subsequent molecular complementation experiments established that the GL2 gene had been cloned. The predicted polypeptide from one of the ORFs contained on this fragment showed significant identity to the homeo domain sequence. The construction of a full-length cDNA by RT-PCR confirmed the presence of a homeo box in the GL2 gene and showed that it is substantially different from other recently cloned homeo box genes in plants. The expression pattern of GL2, as demonstrated by in situ hybridization, indicated that the gene is expressed in trichome progenitor cells and at stages associated with trichome development. This suggests that GL2 may regulate events required for the directional cell expansion observed during trichome formation.

AB - The GLABRA2 gene (GL2) is one of several genes known to have a role in trichome development in Arabidopsis. Mutations at this locus result in abnormal trichome expansion. We have identified several gl2 mutants from a T- DNA-mutagenized population of plants. The T-DNA insert in one of the mutant lines cosegregated with the recessive gl2 phenotype and thus served as a molecular tag to isolate genomic DNA at the putative GL2 locus. RFLP analysis of the segregating population and subsequent molecular complementation experiments established that the GL2 gene had been cloned. The predicted polypeptide from one of the ORFs contained on this fragment showed significant identity to the homeo domain sequence. The construction of a full-length cDNA by RT-PCR confirmed the presence of a homeo box in the GL2 gene and showed that it is substantially different from other recently cloned homeo box genes in plants. The expression pattern of GL2, as demonstrated by in situ hybridization, indicated that the gene is expressed in trichome progenitor cells and at stages associated with trichome development. This suggests that GL2 may regulate events required for the directional cell expansion observed during trichome formation.

KW - Arabidopsis

KW - GLABRA2 gene

KW - homeo box

KW - homeo domain protein

KW - trichome development

UR - http://www.scopus.com/inward/record.url?scp=0028305207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028305207&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 1388

EP - 1399

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 12

ER -