The glossyhead1 allele of acc1 reveals a principal role for multidomain acetyl-coenzyme a carboxylase in the biosynthesis of cuticular waxes by Arabidopsis

Shiyou Lü, Huayan Zhao, Eugene P. Parsons, Changcheng Xu, Dylan K. Kosma, Xiaojing Xu, Daiyin Chao, Gregory Lohrey, Dhinoth K. Bangarusamy, Guangchao Wang, Ray A. Bressan, Matthew A. Jenks

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gene was identified as a new allele of Acetyl-Coenzyme A Carboxylase1 (ACC1), a gene encoding the main enzyme in cytosolic malonyl-coenzyme A synthesis. This, to our knowledge, is the first mutant allele of ACC1 that does not cause lethality at the seed or early germination stage, allowing for the first time a detailed analysis of ACC1 function in mature tissues. Broad lipid profiling of mature gsd1 organs revealed a primary role for ACC1 in the biosynthesis of the very-long-chain fatty acids (C 20:0 or longer) associated with cuticular waxes and triacylglycerols. Unexpectedly, transcriptome analysis revealed that gsd1 has limited impact on any lipid metabolic networks but instead has a large effect on environmental stress-responsive pathways, especially senescence and ethylene synthesis determinants, indicating a possible role for the cytosolic malonyl-coenzyme A-derived lipids in stress response signaling.

Original languageEnglish (US)
Pages (from-to)1079-1092
Number of pages14
JournalPlant physiology
Volume157
Issue number3
DOIs
StatePublished - Nov 2011

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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