The herbicide sensitivity gene CHL1 of arabidopsis encodes a nitrate-inducible nitrate transporter

Yi Fang Tsay, Julian I. Schroeder, Kenneth A Feldmann, Nigel M. Crawford

Research output: Contribution to journalArticle

466 Citations (Scopus)

Abstract

This paper reportsthe identification and functional expression of a gene that is involved in nitrate uptake in plants, a process essential for the assimilation of nitrate and the biological removal of nitrate from the soil solution. The CHL1 gene of Arabldopsis, which when mutated confers resistance to the herbicide chlorate and a decrease in nitrate uptake, was isolated and found to encode a protein with 12 putative membrane-spanning segments. Injection of CHL1 mRNA into Xenopus oocytes produces a nitrate- and pH-dependent membrane depolarization, inward current, and nitrate uptake. These data show that the CHL1 gene encodes an electrogenic nitrate transporter. CHL1 mRNA is found predominantly in roots and displays nitrate- and pH-dependent regulation.

Original languageEnglish (US)
Pages (from-to)705-713
Number of pages9
JournalCell
Volume72
Issue number5
DOIs
StatePublished - Mar 12 1993

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Herbicides
Arabidopsis
Nitrates
Genes
Herbicide Resistance
Chlorates
Membranes
Messenger RNA
Depolarization
Xenopus
nitrate transporters
Oocytes
Soil
Display devices
Soils
Gene Expression
Injections
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The herbicide sensitivity gene CHL1 of arabidopsis encodes a nitrate-inducible nitrate transporter. / Tsay, Yi Fang; Schroeder, Julian I.; Feldmann, Kenneth A; Crawford, Nigel M.

In: Cell, Vol. 72, No. 5, 12.03.1993, p. 705-713.

Research output: Contribution to journalArticle

Tsay, Yi Fang ; Schroeder, Julian I. ; Feldmann, Kenneth A ; Crawford, Nigel M. / The herbicide sensitivity gene CHL1 of arabidopsis encodes a nitrate-inducible nitrate transporter. In: Cell. 1993 ; Vol. 72, No. 5. pp. 705-713.
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