The human thyrotropin β-subunit gene differs in 5' structure from murine TSH-β genes

P. T. Guidon, G Kerr Whitfield, D. Porti, I. A. Kourides

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The gene encoding the β-subunit of human thyrotropin (hTSH-β) was isolated, and its nucleotide sequence was determined. The gene is 4.3 kb in length, consist of three exons and two introns, and is present as a single copy as determined by Southern blot analysis of total genomic DNA. The protein coding portion of the gene, which includes exons 2 and 3, was isolated from a human genomic phage library, while exon 1, which encodes only 5' untranslated mRNA sequence, was isolated from a plasmid library of size-selected genomic DNA fragments. Here we describe the isolation of the 5' untranslated exon of the hTSH-β subunit and 5'-flanking region. The structure of the hTSH-β gene is very similar to the previously characterized TSH-β genes from mouse and rat. The genes from all three species have two distinct promoter regions, but while both promoters are utilized by the murine TSH-β genes, the human TSH-β gene apparently utilizes only the proximal promoter for transcription initiation. A striking difference in hTSH-β gene structure compared to the murine genes is that exon 1 of the human gene is 36 nucleotides. An analysis of the mouse, rat, and human exon 1 and 5'-flanking region shows a high percentage of sequence homology, with the exception of a 9-nucleotide insertion 13 bases 3' from the proximal TATA box found in the human gene but not found in the other two species. We propose that this insertion results in the additional length of human exon 1 compared to the mouse and rat genes. By isolating the promotor region of the hTSH-β gene, we can begin to identify specific sequences involved in the regulation of hTSH gene expression.

Original languageEnglish (US)
Pages (from-to)691-699
Number of pages9
JournalDNA
Volume7
Issue number10
StatePublished - 1988

Fingerprint

Thyrotropin
Genes
Exons
Rats
Nucleotides
5' Flanking Region
Genetic Promoter Regions
TATA Box
Bacteriophages
Gene encoding
DNA
Genomic Library
Transcription
Gene Expression Regulation
Gene expression
Sequence Homology
Southern Blotting
Introns
Plasmids

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Guidon, P. T., Whitfield, G. K., Porti, D., & Kourides, I. A. (1988). The human thyrotropin β-subunit gene differs in 5' structure from murine TSH-β genes. DNA, 7(10), 691-699.

The human thyrotropin β-subunit gene differs in 5' structure from murine TSH-β genes. / Guidon, P. T.; Whitfield, G Kerr; Porti, D.; Kourides, I. A.

In: DNA, Vol. 7, No. 10, 1988, p. 691-699.

Research output: Contribution to journalArticle

Guidon, PT, Whitfield, GK, Porti, D & Kourides, IA 1988, 'The human thyrotropin β-subunit gene differs in 5' structure from murine TSH-β genes', DNA, vol. 7, no. 10, pp. 691-699.
Guidon, P. T. ; Whitfield, G Kerr ; Porti, D. ; Kourides, I. A. / The human thyrotropin β-subunit gene differs in 5' structure from murine TSH-β genes. In: DNA. 1988 ; Vol. 7, No. 10. pp. 691-699.
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