The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

Zênis Novais Da Rocha, Mario Sérgio Pereira Marchesi, Josiane Cristina Molin, Claure N. Lunardi, Katrina M Miranda, Lusiane Maria Bendhack, Peter C. Ford, Roberto Santana Da Silva

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6) 3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around -0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at -0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy) 2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2] +. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L-1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO 2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10-5 mol L-1 of cis-[RuII(NO2)L(bpy)2] + complex.

Original languageEnglish (US)
Pages (from-to)4282-4287
Number of pages6
JournalDalton Transactions
Issue number32
DOIs
StatePublished - 2008

Fingerprint

Nitrites
Ions
Pyrazines
Phenylephrine
Voltammetry
Electrolysis
Cyclic voltammetry
Metal ions
Rats
Ligands
Oxidation
Vasodilation
Sensors
Experiments

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Da Rocha, Z. N., Marchesi, M. S. P., Molin, J. C., Lunardi, C. N., Miranda, K. M., Bendhack, L. M., ... Da Silva, R. S. (2008). The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex. Dalton Transactions, (32), 4282-4287. https://doi.org/10.1039/b803441a

The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex. / Da Rocha, Zênis Novais; Marchesi, Mario Sérgio Pereira; Molin, Josiane Cristina; Lunardi, Claure N.; Miranda, Katrina M; Bendhack, Lusiane Maria; Ford, Peter C.; Da Silva, Roberto Santana.

In: Dalton Transactions, No. 32, 2008, p. 4282-4287.

Research output: Contribution to journalArticle

Da Rocha, ZN, Marchesi, MSP, Molin, JC, Lunardi, CN, Miranda, KM, Bendhack, LM, Ford, PC & Da Silva, RS 2008, 'The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex', Dalton Transactions, no. 32, pp. 4282-4287. https://doi.org/10.1039/b803441a
Da Rocha, Zênis Novais ; Marchesi, Mario Sérgio Pereira ; Molin, Josiane Cristina ; Lunardi, Claure N. ; Miranda, Katrina M ; Bendhack, Lusiane Maria ; Ford, Peter C. ; Da Silva, Roberto Santana. / The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex. In: Dalton Transactions. 2008 ; No. 32. pp. 4282-4287.
@article{d2afba5b592941cc944a13bea74105b2,
title = "The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex",
abstract = "The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6) 3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around -0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at -0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy) 2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2] +. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L-1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO 2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10-5 mol L-1 of cis-[RuII(NO2)L(bpy)2] + complex.",
author = "{Da Rocha}, {Z{\^e}nis Novais} and Marchesi, {Mario S{\'e}rgio Pereira} and Molin, {Josiane Cristina} and Lunardi, {Claure N.} and Miranda, {Katrina M} and Bendhack, {Lusiane Maria} and Ford, {Peter C.} and {Da Silva}, {Roberto Santana}",
year = "2008",
doi = "10.1039/b803441a",
language = "English (US)",
pages = "4282--4287",
journal = "Dalton Transactions",
issn = "1477-9226",
publisher = "Royal Society of Chemistry",
number = "32",

}

TY - JOUR

T1 - The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

AU - Da Rocha, Zênis Novais

AU - Marchesi, Mario Sérgio Pereira

AU - Molin, Josiane Cristina

AU - Lunardi, Claure N.

AU - Miranda, Katrina M

AU - Bendhack, Lusiane Maria

AU - Ford, Peter C.

AU - Da Silva, Roberto Santana

PY - 2008

Y1 - 2008

N2 - The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6) 3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around -0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at -0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy) 2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2] +. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L-1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO 2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10-5 mol L-1 of cis-[RuII(NO2)L(bpy)2] + complex.

AB - The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6) 3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around -0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at -0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy) 2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2] +. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L-1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO 2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10-5 mol L-1 of cis-[RuII(NO2)L(bpy)2] + complex.

UR - http://www.scopus.com/inward/record.url?scp=49149088638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49149088638&partnerID=8YFLogxK

U2 - 10.1039/b803441a

DO - 10.1039/b803441a

M3 - Article

C2 - 18682867

AN - SCOPUS:49149088638

SP - 4282

EP - 4287

JO - Dalton Transactions

JF - Dalton Transactions

SN - 1477-9226

IS - 32

ER -