The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

Zênis Novais Da Rocha, Mario Sérgio Pereira Marchesi, Josiane Cristina Molin, Claure N. Lunardi, Katrina M Miranda, Lusiane Maria Bendhack, Peter C. Ford, Roberto Santana Da Silva

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The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6) 3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around -0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at -0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy) 2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2] +. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L-1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO 2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10-5 mol L-1 of cis-[RuII(NO2)L(bpy)2] + complex.

Original languageEnglish (US)
Pages (from-to)4282-4287
Number of pages6
JournalDalton Transactions
Issue number32
Publication statusPublished - 2008


ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Da Rocha, Z. N., Marchesi, M. S. P., Molin, J. C., Lunardi, C. N., Miranda, K. M., Bendhack, L. M., ... Da Silva, R. S. (2008). The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex. Dalton Transactions, (32), 4282-4287.