The influence of antiadhesion therapies on leukocyte subset accumulation in central nervous system ischemia in rats

Wayne M. Clark, Jeff D. Lauten, Nikola Lessov, William Woodward, Bruce M. Coull

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Although treatment with agents that block leukocyte function, including anti-ICAM-1 and doxycycline, reduces experimental central nervous system (CNS) ischemic injury, it is not known how leukocyte subset accumulation is affected by these agents. Using the rat two-vessel occlusion model and immunohistochemistry, we investigated granulocyte (PMN) and monocyte/macrophage (Mφ) accumulation at 1 and 4 d postischemia. A total of 24 animals were randomized to sham surgery, or to ischemia with saline, anti-ICAM-1, or doxycycline treatments. No leukocytes were observed in sham animals. At 24 h postischemia, there was a moderate infiltration of PMN and Mφ in untreated animals that was significantly decreased with either treatment. At 4 d after ischemia no PMN were identified, with extensive Mφ accumulation occurring in untreated animals that was only partially reduced with doxycycline treatment. These results confirm that both anti-ICAM-1 and doxycycline treatments reduce PMN and Mφ infiltration at 24 h. Delayed Mφ accumulation occurs despite treatment, suggesting that some of these cells represent transformed resident microglia.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalJournal of Molecular Neuroscience
Volume6
Issue number1
DOIs
StatePublished - Mar 1995

Keywords

  • Cerebral ischemia
  • ICAM-1
  • doxycycline
  • granulocytes
  • macrophages

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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