The influence of calcium-free solutions upon permeability characteristics of the rabbit lens

Nicholas A. Delamere, Christopher A. Paterson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Sodium, potassium and chloride content, potential difference and 86Rb efflux rate constants were measured in rabbit lenses bathed in normal or calcium-free EGTA solution. The absence of calcium in the bathing solution resulted in a rapid depolarization of the lens potential, and a two-fold increase in the 68Rb efflux rate. These changes were not due to the alteration of the ion distribution between the lens and its environment in the calcium-free solution, and the increase in the 68Rb efflux rate could be explained on the basis of the depolarization alone. The magnitude of the calcium-free induced depolarization was not diminished when the lens active transport was inhibited by ouabain, but was significantly reduced when part of the sodium in the bathing solution had been replaced by sucrose. These observations suggested that one of the early effects of removal of calcium from the bathing solution is a rapid elevation of lens sodium permeability.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalExperimental eye research
Volume28
Issue number1
DOIs
StatePublished - Jan 1979
Externally publishedYes

Keywords

  • calcium
  • lens
  • permeability
  • potential

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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