The influence of polymer processing methods on polymer film physical properties and vascular cell responsiveness

Kaitlyn R. Ammann, Maxwell Li, Syed Hossainy, Marvin J Slepian

Research output: Contribution to journalArticle

Abstract

Implantable vascular devices typically interface with blood and vascular tissues. Physical properties of device materials and coatings, independent of chemical composition, can significantly influence cell responses and implant success. Here, we analyzed the effect of various polymer processing regimes, using a single implant polymer, poly(ϵ-caprolactone) (PCL), on vascular endothelial cell (EC), smooth muscle cell (SMC), and platelet response. PCL films were formed by varying three parameters: (1) formation method: solvent casting, melt pressing, or spin coating; (2) molecular weight: 50 or 100 kDa; and (3) solvent type: dichloromethane (DCM) or tetrahydrofuran (THF). We quantified the relationship of polymer processing choice to surface roughness, wettability, and bulk stiffness; and to EC adhesion, SMC adhesion, and platelet activity state (PAS). Multiple regression analysis identified which processing method signficantly impacted (F-ratio > p-value; p < 0.1) polymer physical properties and vascular cell interaction. Film formation method affected PCL roughness (Rq), wettability (deg), and stiffness (MPa) with spin coating resulting in the most wettable (81.8 ± 0.7°) and stiffest (1.12 ± 0.07 MPa; p < 0.001) polymer film; however, solvent cast films were the roughest (281 ± 66 nm). Molecular weight influenced wettability, with the highest wettability on 50 kDa films (79.7 ± 0.7° p < 0.001) and DCM solvent films (83.0 ± 1.0° p < 0.01). The multiple regression model confidently predicted (F-ratio = 9.88; p = 0.005) wettability from molecular weight (p = 0.002) and film formation method (p = 0.03); stiffness (F-ratio = 4.21; p = 0.05) also fit well to film formation method (p = 0.02). Film formation method impacted SMC adhesion and platelet activity state, but not EC adhesion, with melt press PCL promoting the highest SMC adhesion (18000 ± 1536 SMCs; p < 0.05) and PAS (5.0 ± 0.7%PAS). The regression model confidently fit SMC adhesion (F-ratio = 3.15; p = 0.09) and PAS (F-ratio = 5.30; p = 0.05) to polymer processing choices, specifically film formation method (p < 0.03). However, only SMC adhesion had a model that fit well (F-ratio = 4.13; p = 0.05) to the physical properties directly, specifically roughness and wettability (p < 0.04).

Original languageEnglish (US)
Pages (from-to)3234-3244
Number of pages11
JournalACS Applied Bio Materials
Volume2
Issue number8
DOIs
StatePublished - Aug 19 2019

Fingerprint

Wettability
Polymer films
Cell Adhesion
Blood Vessels
Cell adhesion
Polymers
Smooth Muscle Myocytes
Physical properties
Platelets
Blood Platelets
Muscle
Wetting
Processing
Endothelial cells
Endothelial Cells
Molecular Weight
Methylene Chloride
Surface roughness
Molecular weight
Stiffness

Keywords

  • biomaterials
  • endothelial cell
  • platelet
  • polymer
  • smooth muscle cell
  • tissue interaction
  • vascular

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

Cite this

The influence of polymer processing methods on polymer film physical properties and vascular cell responsiveness. / Ammann, Kaitlyn R.; Li, Maxwell; Hossainy, Syed; Slepian, Marvin J.

In: ACS Applied Bio Materials, Vol. 2, No. 8, 19.08.2019, p. 3234-3244.

Research output: Contribution to journalArticle

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KW - endothelial cell

KW - platelet

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KW - smooth muscle cell

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