The influence of rearranged T cell receptor αβ transgenes on early thymocyte development

Janko Nikolich-Zugich, Sofija Andjelić, Hung Sia Teh, Nada Jain

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Most murine thymocytes and mature T cells originate from a numerically minor population of CD8-4- (double-negative, DN) thymocytes. In this study, we investigated the effects of rearranged T cell receptor (TcR) α and β transgenes on early T cell development. We analyzed the precursor potential, the expression of CD25 and TcR at mRNA and/or protein level in DN thymocyte subsets in TcR transgenic (Tg) mice. We report the following observations: (i) despite a large overrepresentation of total DN cells in TcR Tg mice, precursor-containing CD25+ DN and CD8Io4Io thymocytes are reduced to a third of the nontransgenic control numbers; (ii) like in the normal mice, CD25+ DN and CD8lo4lo can, and TcR+ DN cells cannot generate other thymic subsets; (iii) TcR α mRNA and TcR α/β protein levels are quantitatively increased, but their developmental expression is similar to that in normal mice; and (iv) surface TcR αβ expression becomes detectable as the thymocytes down-regulate CD25, paralleling the situation in normal mice. Our findings implicate stringent transcriptional control, rather than TcR gene rearrangement, as a decisive regulator of TcR αβ expression in early ontogeny.

Original languageEnglish (US)
Pages (from-to)1699-1704
Number of pages6
JournalEuropean Journal of Immunology
Volume23
Issue number7
StatePublished - Jul 1993
Externally publishedYes

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Thymocytes
T-Cell Antigen Receptor
Transgenes
Transgenic Mice
T-Lymphocyte Gene Rearrangement
T-Lymphocytes
T-Cell Receptor Genes
Messenger RNA
Proteins
Down-Regulation

Keywords

  • Cd84 thymocytes
  • T cell development
  • T cell receptor
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology

Cite this

The influence of rearranged T cell receptor αβ transgenes on early thymocyte development. / Nikolich-Zugich, Janko; Andjelić, Sofija; Teh, Hung Sia; Jain, Nada.

In: European Journal of Immunology, Vol. 23, No. 7, 07.1993, p. 1699-1704.

Research output: Contribution to journalArticle

Nikolich-Zugich, Janko ; Andjelić, Sofija ; Teh, Hung Sia ; Jain, Nada. / The influence of rearranged T cell receptor αβ transgenes on early thymocyte development. In: European Journal of Immunology. 1993 ; Vol. 23, No. 7. pp. 1699-1704.
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