The influence of tryptophan and its metabolites upon rabbit Lens electrolyte balance

K. Kishida, C. A. Paterson, N. A. Delamere

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The effect of tryptophan and three major metabolites of tryptophan on the rabbit lens electrolyte balance was examined. Even at 3 × 10-3 M tryptophan, tryptamine and xanthurenic acid had no effect on lens sodium, potassium and calcium content after a 20 hr incubation. Small but significant changes in lens sodium and potassium content were induced by 20 hr incubation in 2 × 10-3 M 3-hydroxy-DL-kynurenine and 10-3 M hydroxyanthranilic acid. Lens Na,K-ATPase activity was unaffected by 3 × 10-3 M tryptophan. Tryptamine reduced, to a small extent, Na,K-ATPase activity at 3 × 10-3 M, but was without effect at 10-3 M. 3-hydroxy-DL-kynurenine (2 × 10-3 M) and 3-hydroxyanthranilic acid (10-3 M) inhibited Na,K-ATPase activity by 27% and 30% respectively. Only 3-hydroxy-DL-kynurenine had a demonstrable effect on 86Rb uptake. It is concluded that, in contrast to findings in the amphibian lens, metabolites of tryptophan have minimal or no detrimental effects upon rabbit lens electrolyte balance under the in vitro conditions of these experiments. However, this does not rule out a long term effect of these compounds should they accumulate in the lens.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalCurrent Eye Research
Volume2
Issue number5
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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