The binding properties of the calcium antagonist, [3H]nitrendipine, were investigated in homogenates of the rat cerebral cortex and myocardium. The specific component of [3H]nitrendipine binding was consistent with mass action behavior and was described by a high affinity dissociation constant in the range of 0.1 - 0.3 nM. [3H]nitrendipine binding was sensitive to inhibition by other calcium antagonists and by the cations Ca+2 and La+3. The inhibition of [3H]nitrendipine binding by verapamil was not of the simple competitive type but resembled allosteric inhibition, suggesting that there are at least two binding sites for calcium antagonists.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 11 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology