The JHM strain of mouse hepatitis virus induces a spike protein-specific Derestricted cytotoxic T cell response

Cornelia C. Bergmann, Qin Yao, Mark T Lin, Stephen A. Stohlman

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Abstract

Cytotoxic T lymphocyte (CTL) activity specific for mouse hepatitis virus (MHV) JHM strain (JHMV or MHV-4) was examined using in vitro stimulated spleen cells derived from immunized C57BL/6 (H-2b) mice. Target cells infected with JHMV were specifically recognized ; however, analysis of target cells expressing the virus structural proteins via recombinant vaccinia viruses showed no recognition of the viral nucleocapsid (N), membrane (M), small membrane (sM) or haemagglutinin-esterase (HE) proteins. Only target cells expressing the virus spike (S) protein were recognized. Furthermore, the majority of CTL activity was restricted to target cells expressing the MHC class I Db molecules. Analysis of truncations and deletions of the S protein expressed by recombinant vaccinia viruses and peptide coated targets identified a single antigenic epitope, aa 510-518, conforming to the Db binding motif. These amino acids are contained within a domain deleted from a number of strains of mouse hepatitis virus, suggesting a role for immune pressure. To determine the potential for CTL specific for an epitope(s) within a non-structural protein, 24 CTL lines were established and characterized. No evidence for the induction of non-specific CTL activity or virus-specific CTL restricted to an epitope in a non-structural protein was obtained. These data indicate that the predominant CTL activity in JHMV-infected C57BL/6 mice is Db restricted and specific for a single epitope contained within aa 510-518 of the S protein.

Original languageEnglish (US)
Pages (from-to)315-325
Number of pages11
JournalJournal of General Virology
Volume77
Issue number2
StatePublished - 1996
Externally publishedYes

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Murine hepatitis virus
Cytotoxic T-Lymphocytes
T-Lymphocytes
Protein S
Proteins
Epitopes
Vaccinia virus
Viruses
Viral Structural Proteins
Nucleocapsid
T-Lymphocyte Epitopes
Membranes
Inbred C57BL Mouse
Recombinant Proteins
Spleen
Amino Acids
Pressure
Peptides

ASJC Scopus subject areas

  • Immunology
  • Virology

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The JHM strain of mouse hepatitis virus induces a spike protein-specific Derestricted cytotoxic T cell response. / Bergmann, Cornelia C.; Yao, Qin; Lin, Mark T; Stohlman, Stephen A.

In: Journal of General Virology, Vol. 77, No. 2, 1996, p. 315-325.

Research output: Contribution to journalArticle

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