The K/BxN mouse model of inflammatory arthritis: theory and practice.

Paul Monach, Kimie Hattori, Haochu Huang, Elzbieta Hyatt, Jody Morse, Linh Nguyen, Adriana Ortiz-Lopez, Hsin-Jung Joyce Wu, Diane Mathis, Christophe Benoist

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Mice expressing the KRN T cell receptor transgene and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes similar arthritis in a wide range of mouse strains, owing to pathogenic autoantibodies to glucose-6-phosphate isomerase (GPI). This model has been useful for the investigation of the development of autoimmunity (K/BxN transgenic mice) and particularly of the mechanisms by which anti-GPI autoantibodies induce joint-specific imflammation (serum transfer model). In this chaper, after a summary of findings from this model system, we describe detailed methods for the maintenance of a K/BxN colony, crossing of the relevant TCR and MHC genes to other strain backgrounds, evaluation of KRN transgenic T cells, measurement of anti-GPI antibodies, induction of arthritis by serum transfer, and clinical and histological evaluation of arthritis.

Original languageEnglish (US)
Pages (from-to)269-282
Number of pages14
JournalMethods in molecular medicine
Volume136
StatePublished - 2007
Externally publishedYes

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Glucose-6-Phosphate Isomerase
Arthritis
Autoantibodies
Serum
T-Cell Antigen Receptor
Autoimmunity
Transgenes
Transgenic Mice
Joints
Maintenance
T-Lymphocytes
Antibodies
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Monach, P., Hattori, K., Huang, H., Hyatt, E., Morse, J., Nguyen, L., ... Benoist, C. (2007). The K/BxN mouse model of inflammatory arthritis: theory and practice. Methods in molecular medicine, 136, 269-282.

The K/BxN mouse model of inflammatory arthritis : theory and practice. / Monach, Paul; Hattori, Kimie; Huang, Haochu; Hyatt, Elzbieta; Morse, Jody; Nguyen, Linh; Ortiz-Lopez, Adriana; Wu, Hsin-Jung Joyce; Mathis, Diane; Benoist, Christophe.

In: Methods in molecular medicine, Vol. 136, 2007, p. 269-282.

Research output: Contribution to journalArticle

Monach, P, Hattori, K, Huang, H, Hyatt, E, Morse, J, Nguyen, L, Ortiz-Lopez, A, Wu, H-JJ, Mathis, D & Benoist, C 2007, 'The K/BxN mouse model of inflammatory arthritis: theory and practice.', Methods in molecular medicine, vol. 136, pp. 269-282.
Monach P, Hattori K, Huang H, Hyatt E, Morse J, Nguyen L et al. The K/BxN mouse model of inflammatory arthritis: theory and practice. Methods in molecular medicine. 2007;136:269-282.
Monach, Paul ; Hattori, Kimie ; Huang, Haochu ; Hyatt, Elzbieta ; Morse, Jody ; Nguyen, Linh ; Ortiz-Lopez, Adriana ; Wu, Hsin-Jung Joyce ; Mathis, Diane ; Benoist, Christophe. / The K/BxN mouse model of inflammatory arthritis : theory and practice. In: Methods in molecular medicine. 2007 ; Vol. 136. pp. 269-282.
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