The lipid peroxidation end-product and oxidant 4-hydroxynonenal induces insulin resistance in rat slow-twitch skeletal muscle

Mujalin Prasannarong, Fernando R. Santos, Payam Hooshmand, Paria Hooshmand, Franchesca J. Giovannini, Erik J. Henriksen

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Context: The lipid peroxidation end-product and oxidant 4-hydroxynonenal (4-HNE) impairs cell function. However, the impact of 4-HNE on the glucose transport system in mammalian slow-twitch skeletal muscle is not known. Objective: We assessed the effects of 4-HNE on insulin signalling and glucose transport activity in slow-twitch muscle by incubating soleus strips from lean Zucker rats with 4-HNE (50 μM) in the absence or presence of insulin (5 mU/ml) for up to 6 hr. Results: Insulin-stimulated glucose transport activity was significantly (p < 0.05) decreased by 4-HNE at 2 hr. AS160 Thr 642 phosphorylation was decreased at 2 hr, whereas Akt Ser 473 phosphorylation and IRS-1 protein expression were not substantially changed until 4 hr. IRS-2 protein expression was slightly decreased only at 6 hr. Conclusions: The lipid peroxidation end-product and oxidant 4-HNE induces insulin resistance of glucose transport activity in rat slow-twitch skeletal muscle, initially associated with impaired phosphorylation of AS160.

Original languageEnglish (US)
Pages (from-to)22-28
Number of pages7
JournalArchives of Physiology and Biochemistry
Volume120
Issue number1
DOIs
StatePublished - Feb 1 2014

Keywords

  • AS160 phosphorylation
  • Glucose transport
  • Lean Zucker rat
  • Oxidative stress
  • Soleus muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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