The mannose-binding lectin pathway is a significant contributor to reperfusion injury in the type 2 diabetic heart

Laura R. La Bonte, Betsy B Dokken, Grace Davis-Gorman, Gregory L. Stahl, Paul F. McDonagh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The severity of ischaemic heart disease is markedly enhanced in type 2 diabetes. We recently reported that complement activation exacerbates I/R injury in the type 2 diabetic heart. The purpose of this study was to isolate and examine MBL pathway activation following I/R injury in the diabetic heart. ZLC and ZDF rats underwent 30 minutes of left coronary artery occlusion followed by 120 minutes of reperfusion. Two different groups of ZDF rats were treated with either FUT-175, a broad complement inhibitor, or P2D5, a monoclonal antibody raised against rat MBL-A. ZDF rats treated with FUT175 and P2D5 had significantly decreased myocardial infarct size, C3 deposition and neutrophil accumulation compared with untreated ZDF controls. Taken together, these findings indicate that the MBL pathway plays a key role in the severity of complement-mediated I/R injury in the type 2 diabetic heart.

Original languageEnglish (US)
Pages (from-to)172-180
Number of pages9
JournalDiabetes and Vascular Disease Research
Volume6
Issue number3
DOIs
StatePublished - Jul 2009

Fingerprint

Mannose-Binding Lectin
Reperfusion Injury
Complement Inactivating Agents
Wounds and Injuries
Complement Activation
Coronary Occlusion
Type 2 Diabetes Mellitus
Reperfusion
Myocardial Ischemia
Coronary Vessels
Neutrophils
Myocardial Infarction
Monoclonal Antibodies

Keywords

  • Complement
  • Inflammation
  • Ischaemia-reperfusion injury
  • Zucker diabetic fatty rat

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

The mannose-binding lectin pathway is a significant contributor to reperfusion injury in the type 2 diabetic heart. / La Bonte, Laura R.; Dokken, Betsy B; Davis-Gorman, Grace; Stahl, Gregory L.; McDonagh, Paul F.

In: Diabetes and Vascular Disease Research, Vol. 6, No. 3, 07.2009, p. 172-180.

Research output: Contribution to journalArticle

La Bonte, Laura R. ; Dokken, Betsy B ; Davis-Gorman, Grace ; Stahl, Gregory L. ; McDonagh, Paul F. / The mannose-binding lectin pathway is a significant contributor to reperfusion injury in the type 2 diabetic heart. In: Diabetes and Vascular Disease Research. 2009 ; Vol. 6, No. 3. pp. 172-180.
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