The matrix protein CCN1/CYR61 is required for αVβ5-mediated cancer cell migration

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

CYR61 is one of the six proteins of the CCN family of proteins known to play diverse roles in angiogenesis, cellular proliferation, survival, migration and wound healing. However, the specific function of CYR61 in cancer is unclear, and the literature remains controversial. We used quantitative real-time PCR to establish the expression profile of CYR61 and integrin αVβ5 in three non-small cell lung cancer, five colorectal cancer, one breast cancer and one oesophageal squamous carcinoma cell lines. We showed that the levels of CYR61 were significantly increased in oesophageal squamous carcinoma cell line along with the enhanced levels of αVβ5 integrin. Further, we investigated whether tumour cell-secreted CYR61 can facilitate cell migration by interacting with the αVβ5 integrin. Using tumour cell lines with low, intermediate and high CYR61 expression and their isogenic variants as a cellular model, we determined that integrin αVβ5 expressed on these tumour cells is required for cell migration. Moreover, we showed that the modulation of expression levels of CYR61 in these cancer cells affected their capacity for migration. These results represent an advance to the understanding of the role of CYR61 and αvβ5 integrin as proteins that cooperate to mediate cancer cell migration.

Original languageEnglish (US)
Pages (from-to)687-695
Number of pages9
JournalCell Biochemistry and Function
Volume30
Issue number8
DOIs
StatePublished - Dec 2012

Fingerprint

Cysteine-Rich Protein 61
Integrins
Cell Movement
Cells
Tumors
Neoplasms
CCN Intercellular Signaling Proteins
Squamous Cell Carcinoma
Breast Neoplasms
Cell Line
Tumor Cell Line
Non-Small Cell Lung Carcinoma
Wound Healing
Real-Time Polymerase Chain Reaction
Colorectal Neoplasms
Proteins
Cell Proliferation
Modulation

Keywords

  • CYR61
  • Integrin αβ
  • Migration
  • Stable knockdown
  • Stable overexpression

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

The matrix protein CCN1/CYR61 is required for αVβ5-mediated cancer cell migration. / Jandova, Jana; Beyer, Tracey E.; Meuillet, Emmanuelle; Watts, George S.

In: Cell Biochemistry and Function, Vol. 30, No. 8, 12.2012, p. 687-695.

Research output: Contribution to journalArticle

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