The mechanism for the methionine mediated reduction of anthramycin yields in Streptomyces refuineus fermentations

C. Gairola, Laurence Hurley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The addition of L-methionine to cultures of Streptomyces refuineus producing anthramycin, results in an apparent decrease in the antibiotic titer. It has been shown that this effect is not due to the inhibition of tyrosinase, an enzyme implicated in anthramycin biosynthesis. The inhibitory effect also appears unlikely to be due to a regulatory role played by methionine in a non-precursor fashion. Experiments with radiolabeled anthramycin have revealed that the reduced yields of the antibiotic are due to the interaction of anthramycin with generated reactive metabolites produced in the methionine-supplemented cultures.

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalEuropean Journal of Applied Microbiology
Volume2
Issue number2
DOIs
StatePublished - 1976
Externally publishedYes

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Anthramycin
Streptomyces
Antibiotics
Methionine
Fermentation
Enzyme inhibition
Biosynthesis
Metabolites
Enzymes
Anti-Bacterial Agents
Monophenol Monooxygenase
Experiments

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology
  • Microbiology (medical)
  • Medicine(all)

Cite this

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