Abstract
The addition of L-methionine to cultures of Streptomyces refuineus producing anthramycin, results in an apparent decrease in the antibiotic titer. It has been shown that this effect is not due to the inhibition of tyrosinase, an enzyme implicated in anthramycin biosynthesis. The inhibitory effect also appears unlikely to be due to a regulatory role played by methionine in a non-precursor fashion. Experiments with radiolabeled anthramycin have revealed that the reduced yields of the antibiotic are due to the interaction of anthramycin with generated reactive metabolites produced in the methionine-supplemented cultures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-101 |
| Number of pages | 7 |
| Journal | European Journal of Applied Microbiology |
| Volume | 2 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1976 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Microbiology
- Microbiology (medical)
- Medicine(all)
Cite this
The mechanism for the methionine mediated reduction of anthramycin yields in Streptomyces refuineus fermentations. / Gairola, C.; Hurley, Laurence.
In: European Journal of Applied Microbiology, Vol. 2, No. 2, 1976, p. 95-101.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The mechanism for the methionine mediated reduction of anthramycin yields in Streptomyces refuineus fermentations
AU - Gairola, C.
AU - Hurley, Laurence
PY - 1976
Y1 - 1976
N2 - The addition of L-methionine to cultures of Streptomyces refuineus producing anthramycin, results in an apparent decrease in the antibiotic titer. It has been shown that this effect is not due to the inhibition of tyrosinase, an enzyme implicated in anthramycin biosynthesis. The inhibitory effect also appears unlikely to be due to a regulatory role played by methionine in a non-precursor fashion. Experiments with radiolabeled anthramycin have revealed that the reduced yields of the antibiotic are due to the interaction of anthramycin with generated reactive metabolites produced in the methionine-supplemented cultures.
AB - The addition of L-methionine to cultures of Streptomyces refuineus producing anthramycin, results in an apparent decrease in the antibiotic titer. It has been shown that this effect is not due to the inhibition of tyrosinase, an enzyme implicated in anthramycin biosynthesis. The inhibitory effect also appears unlikely to be due to a regulatory role played by methionine in a non-precursor fashion. Experiments with radiolabeled anthramycin have revealed that the reduced yields of the antibiotic are due to the interaction of anthramycin with generated reactive metabolites produced in the methionine-supplemented cultures.
UR - http://www.scopus.com/inward/record.url?scp=0017249046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017249046&partnerID=8YFLogxK
U2 - 10.1007/BF00942352
DO - 10.1007/BF00942352
M3 - Article
AN - SCOPUS:0017249046
VL - 2
SP - 95
EP - 101
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -