The metal efflux island of Legionella pneumophila is not required for survival in macrophages and amoebas

Eun Hae Kim, Xavier Charpentier, Oscar Torres-Urquidy, Megan McEvoy, Christopher Rensing

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Legionella pneumophila is an intracellular pathogen causing pneumonia-like disease in humans. A 43-kb putative heavy metal efflux gene island was found on the L. pneumophila genome. Large Legionella deletion strains of the metal efflux genes were tested in human THP-1-derived macrophages and amoebal Acanthamoeba castellanii cells and were able to survive and replicate similar to the wild type, suggesting that they do not play a significant role within the intracellular environment. Examination of the sequence of this genomic island revealed that some genes were not accurately annotated and there were no known metal-responsive regulators encoded in this region. Therefore, functional roles of these metal resistance genes were tested by conducting metal resistance assays. Individual genes were cloned in an expression vector and expressed in an appropriate metal-sensitive Escherichia coli background with varying concentrations of the tested metal. Of the 11 efflux systems, a role was determined only for one. A Cu(I)-translocating PIB-type ATPase was shown to be encoded by lpg1024. This gene, termed copA, complemented a copper-sensitive (ΔcopA) E. coli strain in trans and was able to confer copper resistance.

Original languageEnglish (US)
Pages (from-to)164-170
Number of pages7
JournalFEMS Microbiology Letters
Volume301
Issue number2
DOIs
StatePublished - Dec 2009

Fingerprint

Legionella pneumophila
Amoeba
Islands
Metals
Macrophages
Survival
Genes
Copper
Acanthamoeba castellanii
Escherichia coli
Genomic Islands
Legionella
Heavy Metals
Adenosine Triphosphatases
Pneumonia
Genome

Keywords

  • Copper efflux
  • Intracellular survival
  • Legionella pneumophila
  • Metal resistance

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

The metal efflux island of Legionella pneumophila is not required for survival in macrophages and amoebas. / Kim, Eun Hae; Charpentier, Xavier; Torres-Urquidy, Oscar; McEvoy, Megan; Rensing, Christopher.

In: FEMS Microbiology Letters, Vol. 301, No. 2, 12.2009, p. 164-170.

Research output: Contribution to journalArticle

Kim, Eun Hae ; Charpentier, Xavier ; Torres-Urquidy, Oscar ; McEvoy, Megan ; Rensing, Christopher. / The metal efflux island of Legionella pneumophila is not required for survival in macrophages and amoebas. In: FEMS Microbiology Letters. 2009 ; Vol. 301, No. 2. pp. 164-170.
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