The MICELI (MICrofluidic, ELectrical, impedance): Prototyping a point-of-care impedance platelet aggregometer

Yana Roka-Moiia, Silvia Bozzi, Chiara Ferrari, Gabriele Mantica, Annalisa Dimasi, Marco Rasponi, Andrea Santoleri, Mariangela Scavone, Filippo Consolo, Marco Cattaneo, Marvin J. Slepian, Alberto Redaelli

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

As key cellular elements of hemostasis, platelets represent a primary target for thrombosis and bleeding management. Currently, therapeutic manipulations of platelet function (antithrombotic drugs) and count (platelet transfusion) are performed with limited or no real-time monitoring of the desired outcome at the point-of-care. To address the need, we have designed and fabricated an easy-to-use, accurate, and portable impedance aggregometer called “MICELI” (MICrofluidic, ELectrical, Impedance). It improves on current platelet aggregation technology by decreasing footprint, assay complexity, and time to obtain results. The current study aimed to optimize the MICELI protocol; validate sensitivity to aggregation agonists and key blood parameters, i.e., platelet count and hematocrit; and verify the MICELI operational performance as compared to commercial impedance aggregometry. We demonstrated that the MICELI aggregometer could detect platelet aggregation in 250 µL of whole blood or platelet-rich plasma, stimulated by ADP, TRAP-6, collagen, epinephrine, and calcium ionophore. Using hirudin as blood anticoagulant allowed higher aggregation values. Aggregation values obtained by the MICELI strongly correlated with platelet count and were not affected by hematocrit. The operational performance comparison of the MICELI and the Multiplate® Analyzer demonstrated strong correlation and similar interdonor distribution of aggregation values obtained between these devices. With the proven reliability of the data obtained by the MICELI aggregometer, it can be further translated into a point-of-care diagnostic device aimed at monitoring platelet function in order to guide pharmacological hemostasis management and platelet transfusions.

Original languageEnglish (US)
Article number1174
JournalInternational journal of molecular sciences
Volume21
Issue number4
DOIs
StatePublished - Feb 2 2020

Keywords

  • Electrical impedance
  • Platelet aggregation
  • Platelet function testing
  • Point-of-care
  • Whole blood aggregometry

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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  • Cite this

    Roka-Moiia, Y., Bozzi, S., Ferrari, C., Mantica, G., Dimasi, A., Rasponi, M., Santoleri, A., Scavone, M., Consolo, F., Cattaneo, M., Slepian, M. J., & Redaelli, A. (2020). The MICELI (MICrofluidic, ELectrical, impedance): Prototyping a point-of-care impedance platelet aggregometer. International journal of molecular sciences, 21(4), [1174]. https://doi.org/10.3390/ijms21041174