The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley

Fusheng Wei, Karin Gobelman-Werner, Shaun M. Morroll, Joachim Kurth, Long Mao, Rod A Wing, Dario Leister, Paul Schulze-Lefert, Roger P. Wise

Research output: Contribution to journalArticle

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Abstract

Powdery mildew of barley, caused by Erysiphe graminis f. sp. hordei, is a model system for investigating the mechanism of gene-for-gene interaction between large-genome cereals and obligate-fungal pathogens. A large number of loci that confer resistance to this disease are located on the short arm of chromosome 5(1H). The Mla resistance-gene cluster is positioned near the telomeric end of this chromosome arm. AFLP-, RAPD-, and RFLP-derived markers were used to saturate the Mla region in a high-resolution recombinant population segregating for the (Mla6 + Mla14) and (Mla13 + Ml-Ru3) resistance specificities. These tightly linked genetic markers were used to identify and develop a physical contig of YAC and BAC clones spanning the Mla cluster. Three distinct NBS-LRR resistance-gene homologue (RGH) families were revealed via computational analysis of low-pass and BAC-end sequence data derived from Mla-spanning clones. Genetic and physical mapping delimited the Mla-associated, NBS-LRR gene families to a 240-kb interval. Recombination within the RGH families was at least 10-fold less frequent than between markers directly adjacent to the Mla cluster.

Original languageEnglish (US)
Pages (from-to)1929-1948
Number of pages20
JournalGenetics
Volume153
Issue number4
StatePublished - Dec 1999
Externally publishedYes

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Hordeum
Genetic Recombination
Chromosomes
DNA
Genes
Physical Chromosome Mapping
Clone Cells
Chromosomes, Human, Pair 5
Disease Resistance
Multigene Family
Genetic Markers
Restriction Fragment Length Polymorphisms
Genome
Population

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley. / Wei, Fusheng; Gobelman-Werner, Karin; Morroll, Shaun M.; Kurth, Joachim; Mao, Long; Wing, Rod A; Leister, Dario; Schulze-Lefert, Paul; Wise, Roger P.

In: Genetics, Vol. 153, No. 4, 12.1999, p. 1929-1948.

Research output: Contribution to journalArticle

Wei, F, Gobelman-Werner, K, Morroll, SM, Kurth, J, Mao, L, Wing, RA, Leister, D, Schulze-Lefert, P & Wise, RP 1999, 'The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley', Genetics, vol. 153, no. 4, pp. 1929-1948.
Wei, Fusheng ; Gobelman-Werner, Karin ; Morroll, Shaun M. ; Kurth, Joachim ; Mao, Long ; Wing, Rod A ; Leister, Dario ; Schulze-Lefert, Paul ; Wise, Roger P. / The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley. In: Genetics. 1999 ; Vol. 153, No. 4. pp. 1929-1948.
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