The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

Paul A Krieg, Susan M. Varnum, W. Michael Wormington, Doug A. Melton

Research output: Contribution to journalArticle

338 Citations (Scopus)

Abstract

A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-α (EF-1α) is synthesized in very large amounts in the early embryo. Transcription of EF-1α mRNA commences at the midblastula transition (MBT), and new EF-1α protein is synthesized very soon after this, as determined by the association of EF-1α mRNA with polysomes. The nucleotide sequence of a full-length EF-1α cDNA clone and the deduced amino acid sequence of Xenopus EF-1α protein are presented.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalDevelopmental Biology
Volume133
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Peptide Elongation Factor 1
Xenopus
Messenger RNA
Gastrula
Polyribosomes
Xenopus laevis
Protein Biosynthesis
Gene Library
Amino Acid Sequence
Proteins
Transcription Factors
Embryonic Structures
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus. / Krieg, Paul A; Varnum, Susan M.; Wormington, W. Michael; Melton, Doug A.

In: Developmental Biology, Vol. 133, No. 1, 1989, p. 93-100.

Research output: Contribution to journalArticle

Krieg, Paul A ; Varnum, Susan M. ; Wormington, W. Michael ; Melton, Doug A. / The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus. In: Developmental Biology. 1989 ; Vol. 133, No. 1. pp. 93-100.
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