The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

Paul A. Krieg, Susan M. Varnum, W. Michael Wormington, Doug A. Melton

Research output: Contribution to journalArticle

338 Scopus citations

Abstract

A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-α (EF-1α) is synthesized in very large amounts in the early embryo. Transcription of EF-1α mRNA commences at the midblastula transition (MBT), and new EF-1α protein is synthesized very soon after this, as determined by the association of EF-1α mRNA with polysomes. The nucleotide sequence of a full-length EF-1α cDNA clone and the deduced amino acid sequence of Xenopus EF-1α protein are presented.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalDevelopmental biology
Volume133
Issue number1
DOIs
StatePublished - May 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus'. Together they form a unique fingerprint.

  • Cite this