The mRNA Export Factor Gle1 and Inositol Hexakisphosphate Regulate Distinct Stages of Translation

Timothy A Bolger, Andrew W. Folkmann, Elizabeth J. Tran, Susan R. Wente

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Gene expression requires proper messenger RNA (mRNA) export and translation. However, the functional links between these consecutive steps have not been fully defined. Gle1 is an essential, conserved mRNA export factor whose export function is dependent on the small molecule inositol hexakisphosphate (IP6). Here, we show that both Gle1 and IP6 are required for efficient translation termination in Saccharomyces cerevisiae and that Gle1 interacts with termination factors. In addition, Gle1 has a conserved physical association with the initiation factor eIF3, and gle1 mutants display genetic interactions with the eIF3 mutant nip1-1. Strikingly, gle1 mutants have defects in initiation, whereas strains lacking IP6 do not. We propose that Gle1 functions together with IP6 and the DEAD-box protein Dbp5 to regulate termination. However, Gle1 also independently mediates initiation. Thus, Gle1 is uniquely positioned to coordinate the mRNA export and translation mechanisms. These results directly impact models for perturbation of Gle1 function in pathophysiology.

Original languageEnglish (US)
Pages (from-to)624-633
Number of pages10
JournalCell
Volume134
Issue number4
DOIs
StatePublished - Aug 22 2008
Externally publishedYes

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Phytic Acid
Messenger RNA
Peptide Initiation Factors
Gene expression
Yeast
Saccharomyces cerevisiae
Gene Expression
Defects
Molecules
Proteins

Keywords

  • CELLBIO
  • PROTEINS
  • RNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The mRNA Export Factor Gle1 and Inositol Hexakisphosphate Regulate Distinct Stages of Translation. / Bolger, Timothy A; Folkmann, Andrew W.; Tran, Elizabeth J.; Wente, Susan R.

In: Cell, Vol. 134, No. 4, 22.08.2008, p. 624-633.

Research output: Contribution to journalArticle

Bolger, Timothy A ; Folkmann, Andrew W. ; Tran, Elizabeth J. ; Wente, Susan R. / The mRNA Export Factor Gle1 and Inositol Hexakisphosphate Regulate Distinct Stages of Translation. In: Cell. 2008 ; Vol. 134, No. 4. pp. 624-633.
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