The N. gonorrhoeae type IV pilus stimulates mechanosensitive pathways and cytoprotection through a pilT-dependent mechanism

Heather L. Howie, Michael Glogauer, Magdalene "Maggie" So

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The Neisseria gonorrhoeae type IV pilus is a retractile appendage that can generate forces near 100 pN. We tested the hypothesis that type IV pilus retraction influences epithelial cell gene expression by exerting tension on the host membrane. Wild-type and retraction-defective bacteria altered the expression of an identical set of epithelial cell genes during attachment. Interestingly, pilus retraction, per se, did not regulate novel gene expression but, rather, enhanced the expression of a subset of the infection-regulated genes. This is accomplished through mitogen-activated protein kinase activation and at least one other undefined stress-activated pathway. These results can be reproduced by applying artificial force on the epithelial membrane, using a magnet and magnetic beads. Importantly, this retraction-mediated signaling increases the ability of the cell to withstand apoptotic signals triggered by infection. We conclude that pilus retraction stimulates mechanosensitive pathways that enhance the expression of stress-responsive genes and activate cytoprotective signaling. A model for the role of pilus retraction in influencing host cell survival is presented.

Original languageEnglish (US)
Pages (from-to)627-637
Number of pages11
JournalPLoS Biology
Volume3
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

Fingerprint

Cytoprotection
Gonorrhea
fimbriae
Genes
Gene expression
Epithelial Cells
Membranes
Gene Expression
Magnets
Neisseria gonorrhoeae
Infection
Mitogen-Activated Protein Kinases
epithelial cells
Cell Survival
Bacteria
gene expression
Chemical activation
Cells
genes
appendages

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

The N. gonorrhoeae type IV pilus stimulates mechanosensitive pathways and cytoprotection through a pilT-dependent mechanism. / Howie, Heather L.; Glogauer, Michael; So, Magdalene "Maggie".

In: PLoS Biology, Vol. 3, No. 4, 04.2005, p. 627-637.

Research output: Contribution to journalArticle

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