The NAD+ precursors, nicotinic acid and nicotinamide upregulate glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase mRNA in Jurkat cells

Qing Yan, Margaret Briehl, Cara L. Crowley, Claire M. Payne, Harris Bernstein, Carol Bernstein

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

To better understand the role of nicotinic acid and nicotinamide in the regulation of the oxidative stress response, we measured the levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (GGPD) mRNA in Jurkat cells treated with these NAD+ precursors. We used a modified nonradioactive Northern blot method and detected the mRNA using 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observed increased levels of the mRNAs for the two enzymes in treated cells. Our findings suggest that the NAD+ precursors may protect against oxidative stress and DNA damage by up-regulating the stress response genes GAPDH and G6PD.

Original languageEnglish (US)
Pages (from-to)133-136
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number1
DOIs
StatePublished - Feb 5 1999

Keywords

  • Glucose-6-phosphate dehydrogenase
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Nicotinamide
  • Nicotinic acid
  • Oxidative stress

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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