The NAD+ precursors, nicotinic acid and nicotinamide upregulate glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase mRNA in Jurkat cells

Qing Yan, Margaret Briehl, Cara L. Crowley, Claire M. Payne, Harris Bernstein, Carol Bernstein

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

To better understand the role of nicotinic acid and nicotinamide in the regulation of the oxidative stress response, we measured the levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (GGPD) mRNA in Jurkat cells treated with these NAD+ precursors. We used a modified nonradioactive Northern blot method and detected the mRNA using 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observed increased levels of the mRNAs for the two enzymes in treated cells. Our findings suggest that the NAD+ precursors may protect against oxidative stress and DNA damage by up-regulating the stress response genes GAPDH and G6PD.

Original languageEnglish (US)
Pages (from-to)133-136
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number1
DOIs
StatePublished - Feb 5 1999

Keywords

  • Glucose-6-phosphate dehydrogenase
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Nicotinamide
  • Nicotinic acid
  • Oxidative stress

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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