The NAD+ precursors, nicotinic acid and nicotinamide upregulate glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase mRNA in Jurkat cells

Qing Yan, Margaret M Briehl, Cara L. Crowley, Claire M. Payne, Harris Bernstein, Carol Bernstein

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

To better understand the role of nicotinic acid and nicotinamide in the regulation of the oxidative stress response, we measured the levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (GGPD) mRNA in Jurkat cells treated with these NAD+ precursors. We used a modified nonradioactive Northern blot method and detected the mRNA using 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observed increased levels of the mRNAs for the two enzymes in treated cells. Our findings suggest that the NAD+ precursors may protect against oxidative stress and DNA damage by up-regulating the stress response genes GAPDH and G6PD.

Original languageEnglish (US)
Pages (from-to)133-136
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number1
DOIs
StatePublished - Feb 5 1999

Fingerprint

Glyceraldehyde-3-Phosphate Dehydrogenases
Jurkat Cells
Niacinamide
Glucosephosphate Dehydrogenase
Niacin
NAD
Oxidative stress
Up-Regulation
Messenger RNA
Oxidative Stress
Digoxigenin
Oligonucleotide Probes
Oligonucleotides
Northern Blotting
DNA Damage
Genes
Cells
DNA
Enzymes

Keywords

  • Glucose-6-phosphate dehydrogenase
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Nicotinamide
  • Nicotinic acid
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The NAD+ precursors, nicotinic acid and nicotinamide upregulate glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase mRNA in Jurkat cells. / Yan, Qing; Briehl, Margaret M; Crowley, Cara L.; Payne, Claire M.; Bernstein, Harris; Bernstein, Carol.

In: Biochemical and Biophysical Research Communications, Vol. 255, No. 1, 05.02.1999, p. 133-136.

Research output: Contribution to journalArticle

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