The NatA acetyltransferase couples sup35 prion complexes to the [PSI +] Phenotype

John A. Pezza, Sara X. Langseth, Rochele Raupp Yamamoto, Stephen M. Doris, Samuel P. Ulin, Arthur R. Salomon, Tricia R Serio

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Protein-only (prion) epigenetic elements confer unique phenotypes by adopting alternate conformations that specify new traits. Given the conformational flexibility of prion proteins, protein-only inheritance requires efficient self-replication of the underlying conformation. To explore the cellular regulation of conformational self-replication and its phenotypic effects, we analyzed genetic interactions between [PSI +], a prion form of the S. cerevisiae Sup35 protein (Sup35 PSI+), and the three N a-acetyltransferases, NatA, NatB, and NatC, which collectively modify ∼50% of yeast proteins. Although prion propagation proceeds normally in the absence of NatB or NatC, the [PSI +] phenotype is reversed in strains lacking NatA. Despite this change in phenotype, [PSI +] NatA mutants continue to propagate heritable Sup35 [PSI+]. This uncoupling of protein state and phenotype does not arise through a decrease in the number or activity of prion templates (propagons) or through an increase in soluble Sup35. Rather, NatA null strains are specifically impaired in establishing the translation termination defect that normally accompanies Sup35 incorporation into prion complexes. The NatA effect cannot be explained by the modification of known components of the [PSI +] prion cycle including Sup35; thus, novel acetylated cellular factors must act to establish and maintain the tight link between Sup35 [PSI+] complexes and their phenotypic effects.

Original languageEnglish (US)
Pages (from-to)1068-1080
Number of pages13
JournalMolecular Biology of the Cell
Volume20
Issue number3
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Acetyltransferases
Phenotype
Proteins
Saccharomyces cerevisiae Proteins
Fungal Proteins
Epigenomics

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Pezza, J. A., Langseth, S. X., Yamamoto, R. R., Doris, S. M., Ulin, S. P., Salomon, A. R., & Serio, T. R. (2009). The NatA acetyltransferase couples sup35 prion complexes to the [PSI +] Phenotype. Molecular Biology of the Cell, 20(3), 1068-1080. https://doi.org/10.1091/mbc.E08-04-0436

The NatA acetyltransferase couples sup35 prion complexes to the [PSI +] Phenotype. / Pezza, John A.; Langseth, Sara X.; Yamamoto, Rochele Raupp; Doris, Stephen M.; Ulin, Samuel P.; Salomon, Arthur R.; Serio, Tricia R.

In: Molecular Biology of the Cell, Vol. 20, No. 3, 01.01.2009, p. 1068-1080.

Research output: Contribution to journalArticle

Pezza, JA, Langseth, SX, Yamamoto, RR, Doris, SM, Ulin, SP, Salomon, AR & Serio, TR 2009, 'The NatA acetyltransferase couples sup35 prion complexes to the [PSI +] Phenotype', Molecular Biology of the Cell, vol. 20, no. 3, pp. 1068-1080. https://doi.org/10.1091/mbc.E08-04-0436
Pezza JA, Langseth SX, Yamamoto RR, Doris SM, Ulin SP, Salomon AR et al. The NatA acetyltransferase couples sup35 prion complexes to the [PSI +] Phenotype. Molecular Biology of the Cell. 2009 Jan 1;20(3):1068-1080. https://doi.org/10.1091/mbc.E08-04-0436
Pezza, John A. ; Langseth, Sara X. ; Yamamoto, Rochele Raupp ; Doris, Stephen M. ; Ulin, Samuel P. ; Salomon, Arthur R. ; Serio, Tricia R. / The NatA acetyltransferase couples sup35 prion complexes to the [PSI +] Phenotype. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 3. pp. 1068-1080.
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