The natural killer cell-like lytic activity expressed by cytolytic T lymphocytes is associated with the expression of a novel function-associated molecule

David T. Harris, L. Jaso-Friedmann, R. B. Devlin, H. S. Koren, D. L. Evans

Research output: Contribution to journalArticle

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Abstract

Experiments were formed to analyse the natural killer cell (NK)-like cytotoxicity frequently expressed by human antigen-specific cytolytic T lymphocytes (CTL). To this end, several monoclonal antibodies (MoAbs) previously shown to identify a novel function-associated molecule (FAM) involved in human NK function were utilized. Flow cytometry revealed that these MoAbs reacted with the majority of human NK, but only with a subpopulation of CTL isolated from primary mixed lymphocyte cultures. Preincubation of CTL with the MoAbs inhibited the NK-like lysis of K562 targets. Experiments with anti-CD3 MoAb demonstrated that neither the NK-like cytotoxicity of CTL nor the lytic activity of NK were mediated by the CD3 complex. Expression of the novel FAM was found to develop in T-cell cultures at the time that NK-like cytotoxicity was observed. Repeated in vitro antigenic stimulation of CTL was shown to result in loss of NK-like cytotoxicity, as well as loss of the FAM on the CTL surface. Thus, NK-like cytotoxicity displayed by antigen-specific CTL appears to be mediated by a novel FAM that is identical to that structure found on NK.

Original languageEnglish (US)
Pages (from-to)299-309
Number of pages11
JournalScandinavian Journal of Immunology
Volume35
Issue number3
StatePublished - 1992

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Natural Killer Cells
T-Lymphocytes
Monoclonal Antibodies
CD3 Antigens
Antigens
Flow Cytometry
Cell Culture Techniques
Lymphocytes

ASJC Scopus subject areas

  • Immunology

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The natural killer cell-like lytic activity expressed by cytolytic T lymphocytes is associated with the expression of a novel function-associated molecule. / Harris, David T.; Jaso-Friedmann, L.; Devlin, R. B.; Koren, H. S.; Evans, D. L.

In: Scandinavian Journal of Immunology, Vol. 35, No. 3, 1992, p. 299-309.

Research output: Contribution to journalArticle

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