The nitroxyl donor Angeli's salt ameliorates Staphylococcus aureus-induced septic arthritis in mice

Larissa Staurengo-Ferrari, Kenji W. Ruiz-Miyazawa, Felipe A. Pinho-Ribeiro, Talita P. Domiciano, Victor Fattori, Sandra S. Mizokami, Jacinta S. Pelayo, Juliano Bordignon, Florêncio Figueiredo, Rubia Casagrande, Katrina M Miranda, Waldiceu A. Verri

Research output: Contribution to journalArticle

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Abstract

Septic arthritis is a severe and rapidly debilitating disease associated with severe joint pain, inflammation and oxidative stress. Nitroxyl (HNO) has become a nitrogen oxide of significant interest due to its pharmacological endpoints that are potentially favorable for treating varied diseases. However, whether HNO also serves as a treatment to septic arthritis is currently unknown. The aim of this study was to investigate the effect of the HNO donor, Angeli's salt (AS), in the outcome of chronic Staphylococcus aureus (S. aureus)-induced septic arthritis in mice. Daily treatment with AS inhibited mechanical hyperalgesia and inflammation (edema, leukocyte migration, cytokines release and NF-κB activation, and oxidative stress) resulting in reduced disease severity (clinical course, histopathological changes, proteoglycan levels in the joints, and osteoclastogenesis). In addition, AS decreased the number of S. aureus colony forming unities in synovial tissue, enhanced the bactericidal effect of macrophages and inhibited the worsening of systemic inflammatory response (leukocyte counts in the lung and systemic proinflammatory cytokine concentration). Our results suggest for the first time the therapeutic potential of AS in a model of septic arthritis by mechanisms involving microbicidal effects, anti-inflammatory actions and reduction of disease severity.

Original languageEnglish (US)
Pages (from-to)487-499
Number of pages13
JournalFree Radical Biology and Medicine
Volume108
DOIs
StatePublished - Jul 1 2017

Fingerprint

Infectious Arthritis
Staphylococcus aureus
Oxidative stress
Oxidative Stress
Cytokines
Inflammation
Macrophages
Hyperalgesia
Arthralgia
Proteoglycans
Leukocyte Count
Osteogenesis
Edema
Nitric Oxide
Leukocytes
Anti-Inflammatory Agents
Therapeutics
Joints
Chemical activation
Pharmacology

Keywords

  • Angeli's salt
  • Cytokines
  • Leukocytes
  • Nitric oxide
  • Nitroxyl
  • Oxidative stress
  • Pain
  • Septic arthritis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Staurengo-Ferrari, L., Ruiz-Miyazawa, K. W., Pinho-Ribeiro, F. A., Domiciano, T. P., Fattori, V., Mizokami, S. S., ... Verri, W. A. (2017). The nitroxyl donor Angeli's salt ameliorates Staphylococcus aureus-induced septic arthritis in mice. Free Radical Biology and Medicine, 108, 487-499. https://doi.org/10.1016/j.freeradbiomed.2017.04.016

The nitroxyl donor Angeli's salt ameliorates Staphylococcus aureus-induced septic arthritis in mice. / Staurengo-Ferrari, Larissa; Ruiz-Miyazawa, Kenji W.; Pinho-Ribeiro, Felipe A.; Domiciano, Talita P.; Fattori, Victor; Mizokami, Sandra S.; Pelayo, Jacinta S.; Bordignon, Juliano; Figueiredo, Florêncio; Casagrande, Rubia; Miranda, Katrina M; Verri, Waldiceu A.

In: Free Radical Biology and Medicine, Vol. 108, 01.07.2017, p. 487-499.

Research output: Contribution to journalArticle

Staurengo-Ferrari, L, Ruiz-Miyazawa, KW, Pinho-Ribeiro, FA, Domiciano, TP, Fattori, V, Mizokami, SS, Pelayo, JS, Bordignon, J, Figueiredo, F, Casagrande, R, Miranda, KM & Verri, WA 2017, 'The nitroxyl donor Angeli's salt ameliorates Staphylococcus aureus-induced septic arthritis in mice', Free Radical Biology and Medicine, vol. 108, pp. 487-499. https://doi.org/10.1016/j.freeradbiomed.2017.04.016
Staurengo-Ferrari L, Ruiz-Miyazawa KW, Pinho-Ribeiro FA, Domiciano TP, Fattori V, Mizokami SS et al. The nitroxyl donor Angeli's salt ameliorates Staphylococcus aureus-induced septic arthritis in mice. Free Radical Biology and Medicine. 2017 Jul 1;108:487-499. https://doi.org/10.1016/j.freeradbiomed.2017.04.016
Staurengo-Ferrari, Larissa ; Ruiz-Miyazawa, Kenji W. ; Pinho-Ribeiro, Felipe A. ; Domiciano, Talita P. ; Fattori, Victor ; Mizokami, Sandra S. ; Pelayo, Jacinta S. ; Bordignon, Juliano ; Figueiredo, Florêncio ; Casagrande, Rubia ; Miranda, Katrina M ; Verri, Waldiceu A. / The nitroxyl donor Angeli's salt ameliorates Staphylococcus aureus-induced septic arthritis in mice. In: Free Radical Biology and Medicine. 2017 ; Vol. 108. pp. 487-499.
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