The novel guanylyl cyclase MsGC-I is strongly expressed in higher-order neuropils in the brain of Manduca sexta

Alan J Nighorn, P. J. Simpson, D. B. Morton

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Guanylyl cyclases are usually characterized as being either soluble (sGCs) or receptor (rGCs). We have recently cloned a novel guanylyl cyclase, MsGC-I, from the developing nervous system of the hawkmoth Manduca sexta that cannot be classified as either an sGC or an rGC. MsGC-I shows highest sequence identity with receptor guanylyl cyclases throughout its catalytic and dimerization domains, but does not contain the ligand-binding, transmembrane or kinase-like domains characteristic of receptor guanylyl cyclases. In addition, MsGC-I contains a C-terminal extension of 149 amino acid residues. In this paper, we report the expression of MsGC-I in the adult. Northern blots show that it is expressed preferentially in the nervous system, with high levels in the pharate adult brain and antennae. In the antennae, immunohistochemical analyses show that it is expressed in the cell bodies and dendrites, but not axons, of olfactory receptor neurons. In the brain, it is expressed in a variety of sensory neuropils including the antennal and optic lobes. It is also expressed in structures involved in higher-order processing including the mushroom bodies and central complex. This complicated expression pattern suggests that this novel guanylyl cyclase plays an important role in mediating cyclic GMP levels in the nervous system of Manduca sexta.

Original languageEnglish (US)
Pages (from-to)305-314
Number of pages10
JournalJournal of Experimental Biology
Volume204
Issue number2
StatePublished - 2001

Fingerprint

Manduca
guanylate cyclase
Neuropil
Guanylate Cyclase
Manduca sexta
Guanylate Cyclase-Coupled Receptors
nervous system
Nervous System
brain
antenna
Brain
Olfactory Receptor Neurons
Mushroom Bodies
antennae
Cyclic GMP
receptors
mushroom
Dimerization
Dendrites
Northern Blotting

Keywords

  • Cyclic GMP
  • Guanylyl cyclase
  • Hawkamoth
  • Immunohistochemistry
  • Manduca sexta
  • Mushroom body
  • Olfaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

The novel guanylyl cyclase MsGC-I is strongly expressed in higher-order neuropils in the brain of Manduca sexta. / Nighorn, Alan J; Simpson, P. J.; Morton, D. B.

In: Journal of Experimental Biology, Vol. 204, No. 2, 2001, p. 305-314.

Research output: Contribution to journalArticle

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