The nucleotide sequence and comparative analysis of the H-2D(p) class I H-2 gene

B. S. Schepart, H. Takahashi, K. M. Cozad, R. Murray, K. Ozato, E. Appella, Jeffrey A Frelinger

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We present the complete nucleotide sequence and the deduced amino acid sequence of the H-2D(p) class I gene. This gene, which was cloned from a B10.P genomic DNA library, encodes an intact, functional H-2D(p) molecule. Comparative analysis of the D(p) sequence with other class I sequences reveals both similarities and differences. This analysis also shows that these genes exhibit D region-specific, locus-specific, as well as allele-specific sequences. The H-2D(p) nucleotide sequence is greater than 90% homologous to the H-2L(d) and H-2Db genes and only approximately 85% homologous to the H-2D(d) gene. The K region and Qa region genes are less homologous. The 3' noncoding sequences appear to be region-specific. All of the previously described D region genes, Db, L(d), and D(d), possess the B2-SINE Alu-like repetitive sequences, as does D(p). Thus, this B2 repeat is a region-specific marker present in all D region genes studied so far. The additional poly-adenylation site found in the H-2D(p) gene starting at nucleotide 4671, which is homologous to non-D region sequences, as well as unique protein D(p) coding sequences, make this gene an interesting model for studying the evolution of polymorphism and structure/function relationships in the class I gene family.

Original languageEnglish (US)
Pages (from-to)3489-3495
Number of pages7
JournalJournal of Immunology
Volume136
Issue number9
StatePublished - 1986
Externally publishedYes

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MHC Class I Genes
Sequence Analysis
Genes
Short Interspersed Nucleotide Elements
Alu Elements
Genomic Library
Gene Library
Amino Acid Sequence
Nucleotides
Alleles

ASJC Scopus subject areas

  • Immunology

Cite this

Schepart, B. S., Takahashi, H., Cozad, K. M., Murray, R., Ozato, K., Appella, E., & Frelinger, J. A. (1986). The nucleotide sequence and comparative analysis of the H-2D(p) class I H-2 gene. Journal of Immunology, 136(9), 3489-3495.

The nucleotide sequence and comparative analysis of the H-2D(p) class I H-2 gene. / Schepart, B. S.; Takahashi, H.; Cozad, K. M.; Murray, R.; Ozato, K.; Appella, E.; Frelinger, Jeffrey A.

In: Journal of Immunology, Vol. 136, No. 9, 1986, p. 3489-3495.

Research output: Contribution to journalArticle

Schepart, BS, Takahashi, H, Cozad, KM, Murray, R, Ozato, K, Appella, E & Frelinger, JA 1986, 'The nucleotide sequence and comparative analysis of the H-2D(p) class I H-2 gene', Journal of Immunology, vol. 136, no. 9, pp. 3489-3495.
Schepart BS, Takahashi H, Cozad KM, Murray R, Ozato K, Appella E et al. The nucleotide sequence and comparative analysis of the H-2D(p) class I H-2 gene. Journal of Immunology. 1986;136(9):3489-3495.
Schepart, B. S. ; Takahashi, H. ; Cozad, K. M. ; Murray, R. ; Ozato, K. ; Appella, E. ; Frelinger, Jeffrey A. / The nucleotide sequence and comparative analysis of the H-2D(p) class I H-2 gene. In: Journal of Immunology. 1986 ; Vol. 136, No. 9. pp. 3489-3495.
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