The opaH locus of Neisseria gonorrhoeae MS11A is involved in epithelial cell invasion

Lillian S. Waldbeser, Richard S. Ajioka, Alexey J. Merz, Deidre Puaoi, Lan Lin, Mary Thomas, Magdalene "Maggie" So

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In order to produce a successful infection, Neisseria gonorrhoeae (GC) must attach to and invade mucosal epithelial cells. To identify GC gene products involved in this early interaction with host cells we constructed a gene bank derived from a clinical isolate of GC, and isolated a clone which had the capacity to adhere to the human endometrial adenocarcinoma tissue-culture line HEC-1-B. The cloned sequence was identified as a member of the opa gene family whose protein products have been associated with virulence. The GC chromosome contains numerous variant opa genes which, in MS11, are designated opaA-K Previous work showed that expression of opaC confers a highly invasive phenotype upon strain MS11. When our cloned opa gene was mutated and returned to the GC MS11A chromosome by transformation and homologous recombination, we isolated one transformant that was significantly reduced in its invasive capacity. The locus mutated in this transformant was identified as opaH. Our results indicate that invasiveness of GC for human epithelial cells can be determined by more than one opa gene in strain MS11 A.

Original languageEnglish (US)
Pages (from-to)919-928
Number of pages10
JournalMolecular Microbiology
Volume13
Issue number5
StatePublished - Sep 1994
Externally publishedYes

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Neisseria gonorrhoeae
Epithelial Cells
Genes
Chromosomes
Homologous Recombination
Virulence
Adenocarcinoma
Clone Cells
Phenotype
Infection
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Waldbeser, L. S., Ajioka, R. S., Merz, A. J., Puaoi, D., Lin, L., Thomas, M., & So, M. M. (1994). The opaH locus of Neisseria gonorrhoeae MS11A is involved in epithelial cell invasion. Molecular Microbiology, 13(5), 919-928.

The opaH locus of Neisseria gonorrhoeae MS11A is involved in epithelial cell invasion. / Waldbeser, Lillian S.; Ajioka, Richard S.; Merz, Alexey J.; Puaoi, Deidre; Lin, Lan; Thomas, Mary; So, Magdalene "Maggie".

In: Molecular Microbiology, Vol. 13, No. 5, 09.1994, p. 919-928.

Research output: Contribution to journalArticle

Waldbeser, LS, Ajioka, RS, Merz, AJ, Puaoi, D, Lin, L, Thomas, M & So, MM 1994, 'The opaH locus of Neisseria gonorrhoeae MS11A is involved in epithelial cell invasion', Molecular Microbiology, vol. 13, no. 5, pp. 919-928.
Waldbeser LS, Ajioka RS, Merz AJ, Puaoi D, Lin L, Thomas M et al. The opaH locus of Neisseria gonorrhoeae MS11A is involved in epithelial cell invasion. Molecular Microbiology. 1994 Sep;13(5):919-928.
Waldbeser, Lillian S. ; Ajioka, Richard S. ; Merz, Alexey J. ; Puaoi, Deidre ; Lin, Lan ; Thomas, Mary ; So, Magdalene "Maggie". / The opaH locus of Neisseria gonorrhoeae MS11A is involved in epithelial cell invasion. In: Molecular Microbiology. 1994 ; Vol. 13, No. 5. pp. 919-928.
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