The peripheral myelin protein 22 kDa (PMP22) gene is amplified in cell lines derived from glioma and osteogenic sarcoma: A new candidate for an oncogene?

T. Liehr, O. Park, Burt G.F. Feuerstein, E. Gebhart, B. Rautenstrauss

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two glioma (SF188, SF763) and two osteogenic sarcoma cell lines (RH30, SA1) were examined for the presence of an amplification of the PMP22 gene by means of fluorescence in situ hybridization (FISH). In one cell line of both cell types, we found about 10 copies of the PMP22 (peripheral myelin protein 22 kDa) gene, located on different marker chromosomes within homogeneously staining regions. Surrounding chromosome 17p material was found to be coamplified, but coamplification of TP53 (17p13) and erbB2/Her2 (17q11.12) were excluded by FISH for both cell lines, SF763 and RH30. This is the first report of a PMP22 amplification in cell lines derived from human tumors.

Original languageEnglish (US)
Pages (from-to)915-919
Number of pages5
JournalInternational Journal of Oncology
Volume10
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Myelin Proteins
Osteosarcoma
Oncogenes
Glioma
Cell Line
Fluorescence In Situ Hybridization
Genes
Genetic Markers
Chromosomes
Staining and Labeling
Neoplasms

Keywords

  • amplification
  • FISH
  • glioma
  • oncogene
  • osteogenic sarcoma
  • PMP22

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The peripheral myelin protein 22 kDa (PMP22) gene is amplified in cell lines derived from glioma and osteogenic sarcoma : A new candidate for an oncogene? / Liehr, T.; Park, O.; Feuerstein, Burt G.F.; Gebhart, E.; Rautenstrauss, B.

In: International Journal of Oncology, Vol. 10, No. 5, 1997, p. 915-919.

Research output: Contribution to journalArticle

@article{be2f3b88f016435ab124480d705afc59,
title = "The peripheral myelin protein 22 kDa (PMP22) gene is amplified in cell lines derived from glioma and osteogenic sarcoma: A new candidate for an oncogene?",
abstract = "Two glioma (SF188, SF763) and two osteogenic sarcoma cell lines (RH30, SA1) were examined for the presence of an amplification of the PMP22 gene by means of fluorescence in situ hybridization (FISH). In one cell line of both cell types, we found about 10 copies of the PMP22 (peripheral myelin protein 22 kDa) gene, located on different marker chromosomes within homogeneously staining regions. Surrounding chromosome 17p material was found to be coamplified, but coamplification of TP53 (17p13) and erbB2/Her2 (17q11.12) were excluded by FISH for both cell lines, SF763 and RH30. This is the first report of a PMP22 amplification in cell lines derived from human tumors.",
keywords = "amplification, FISH, glioma, oncogene, osteogenic sarcoma, PMP22",
author = "T. Liehr and O. Park and Feuerstein, {Burt G.F.} and E. Gebhart and B. Rautenstrauss",
year = "1997",
language = "English (US)",
volume = "10",
pages = "915--919",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - The peripheral myelin protein 22 kDa (PMP22) gene is amplified in cell lines derived from glioma and osteogenic sarcoma

T2 - A new candidate for an oncogene?

AU - Liehr, T.

AU - Park, O.

AU - Feuerstein, Burt G.F.

AU - Gebhart, E.

AU - Rautenstrauss, B.

PY - 1997

Y1 - 1997

N2 - Two glioma (SF188, SF763) and two osteogenic sarcoma cell lines (RH30, SA1) were examined for the presence of an amplification of the PMP22 gene by means of fluorescence in situ hybridization (FISH). In one cell line of both cell types, we found about 10 copies of the PMP22 (peripheral myelin protein 22 kDa) gene, located on different marker chromosomes within homogeneously staining regions. Surrounding chromosome 17p material was found to be coamplified, but coamplification of TP53 (17p13) and erbB2/Her2 (17q11.12) were excluded by FISH for both cell lines, SF763 and RH30. This is the first report of a PMP22 amplification in cell lines derived from human tumors.

AB - Two glioma (SF188, SF763) and two osteogenic sarcoma cell lines (RH30, SA1) were examined for the presence of an amplification of the PMP22 gene by means of fluorescence in situ hybridization (FISH). In one cell line of both cell types, we found about 10 copies of the PMP22 (peripheral myelin protein 22 kDa) gene, located on different marker chromosomes within homogeneously staining regions. Surrounding chromosome 17p material was found to be coamplified, but coamplification of TP53 (17p13) and erbB2/Her2 (17q11.12) were excluded by FISH for both cell lines, SF763 and RH30. This is the first report of a PMP22 amplification in cell lines derived from human tumors.

KW - amplification

KW - FISH

KW - glioma

KW - oncogene

KW - osteogenic sarcoma

KW - PMP22

UR - http://www.scopus.com/inward/record.url?scp=0030914187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030914187&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030914187

VL - 10

SP - 915

EP - 919

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 5

ER -