The platelet glycoprotein IIb/IIIa-like protein in human endothelial cells promotes adhesion but not initial attachment to extracellular matrix.

C. S. Chen, P. Thiagarajan, S. M. Schwartz, J. M. Harlan, Ronald L Heimark

Research output: Contribution to journalArticle

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Abstract

On platelets the membrane glycoprotein IIb/IIIa complex (GPIIb/IIIa) functions in adhesive interactions with fibrinogen, von Willebrand factor, and fibronectin. However, the function of GPIIb/IIIa-like proteins on endothelial cells, as well as the ligand(s) the complex binds, is unknown. Using a highly specific polyclonal antibody we have explored the function of GPIIb/IIIa-like proteins on human umbilical vein endothelial cells (HUVE). Analysis by immunoblotting shows that this antiserum recognizes the endothelial GPIIIa-like protein of the complex. The IgG fraction of the polyclonal antiserum and its Fab' fragments detach confluent and subconfluent HUVE from extracellular substrata. The effect of the anti-GPIIb/IIIa IgG is not toxic as the detached cells maintain their viability after trypsinization and replating. Anti-GPIIb/IIIa IgG does not inhibit HUVE binding to extracellular matrix or purified fibronectin in an attachment assay despite the presence of intact GPIIb/IIIa on HUVE detached from substrate by various methods. Apparently, the GPIIb/IIIa-like protein on HUVE is important in normal HUVE adhesion to the extracellular matrix, but it is not required in the initial attachment of HUVE to extracellular matrix.

Original languageEnglish (US)
Pages (from-to)1885-1892
Number of pages8
JournalJournal of Cell Biology
Volume105
Issue number4
StatePublished - Oct 1987
Externally publishedYes

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Integrin beta3
Platelet Glycoprotein GPIIb-IIIa Complex
Human Umbilical Vein Endothelial Cells
Cell Adhesion
Extracellular Matrix
Endothelial Cells
Proteins
Immunoglobulin G
Fibronectins
Immune Sera
Platelet Membrane Glycoprotein IIb
Immunoglobulin Fab Fragments
Poisons
von Willebrand Factor
Immunoblotting
Adhesives
Fibrinogen
Ligands
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

The platelet glycoprotein IIb/IIIa-like protein in human endothelial cells promotes adhesion but not initial attachment to extracellular matrix. / Chen, C. S.; Thiagarajan, P.; Schwartz, S. M.; Harlan, J. M.; Heimark, Ronald L.

In: Journal of Cell Biology, Vol. 105, No. 4, 10.1987, p. 1885-1892.

Research output: Contribution to journalArticle

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