The protozoan parasite Toxoplasma gondii targets proteins to dense granules and the vacuolar space using both conserved and unusual mechanisms

Verena Karsten, Huilin Qi, Con J M Beckers, Anita Reddy, Jean Francois Dubremetz, Paul Webster, Keith A Joiner

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

All known proteins that accumulate in the vacuolar space surrounding the obligate intracellular protozoan parasite Toxoplasma gondii are derived from parasite dense granules. To determine if constitutive secretory vesicles could also mediate delivery to the vacuolar space, T. gondii was stably transfected with soluble Escherichia coli alkaline phosphatase and E. coli β-lactamase. Surprisingly, both foreign secretory reporters were delivered quantitatively into parasite dense granules and efficiently secreted into the vacuolar space. Addition of a glycosylphosphatidylinositol membrane anchor rerouted alkaline phosphatase to the parasite surface. Alkaline phosphatase fused to the transmembrane domain and cytoplasmic tail from the endogenous dense granule protein GRA4 localized to dense granules. The protein was secreted into a tuboreticular network in the vacuolar space, in a fashion dependent upon the cytoplasmic tail, but not upon a tyrosine-based motif within the tail. Alkaline phosphatase fused to the vesicular stomatitis virus G protein transmembrane domain and cytoplasmic tail localized primarily to the Golgi, although staining of dense granules and the intravacuolar network was also detected; truncating the cytoplasmic tail decreased Golgi staining and increased delivery to dense granules but blocked delivery to the intravacuolar network. Targeting of secreted proteins to T. gondii dense granules and the plasma membrane uses general mechanisms identified in higher eukaryotic cells but is simplified and exaggerated in scope, while targeting of secreted proteins beyond the boundaries of the parasite involves unusual sorting events.

Original languageEnglish (US)
Pages (from-to)1323-1333
Number of pages11
JournalJournal of Cell Biology
Volume141
Issue number6
DOIs
StatePublished - Jun 15 1998
Externally publishedYes

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Toxoplasma
Tail
Parasites
Alkaline Phosphatase
Protein Transport
Proteins
Staining and Labeling
Escherichia coli
Glycosylphosphatidylinositols
Secretory Vesicles
Eukaryotic Cells
Tyrosine
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

The protozoan parasite Toxoplasma gondii targets proteins to dense granules and the vacuolar space using both conserved and unusual mechanisms. / Karsten, Verena; Qi, Huilin; Beckers, Con J M; Reddy, Anita; Dubremetz, Jean Francois; Webster, Paul; Joiner, Keith A.

In: Journal of Cell Biology, Vol. 141, No. 6, 15.06.1998, p. 1323-1333.

Research output: Contribution to journalArticle

Karsten, Verena ; Qi, Huilin ; Beckers, Con J M ; Reddy, Anita ; Dubremetz, Jean Francois ; Webster, Paul ; Joiner, Keith A. / The protozoan parasite Toxoplasma gondii targets proteins to dense granules and the vacuolar space using both conserved and unusual mechanisms. In: Journal of Cell Biology. 1998 ; Vol. 141, No. 6. pp. 1323-1333.
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