The Pseudomonas viridiflava phylogroups in the P.syringae species complex are characterized by genetic variability and phenotypic plasticity of pathogenicity-related traits

Claudia Bartoli, Odile Berge, Caroline L. Monteil, Caroline Guilbaud, Giorgio M. Balestra, Leonardo Varvaro, Corbin Jones, Jeffery L. Dangl, David A Baltrus, David C. Sands, Cindy E. Morris

Research output: Contribution to journalArticle

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Abstract

As a species complex, Pseudomonas syringae exists in both agriculture and natural aquatic habitats. P.viridiflava, a member of this complex, has been reported to be phenotypically largely homogenous. We characterized strains from different habitats, selected based on their genetic similarity to previously described P.viridiflava strains. We revealed two distinct phylogroups and two different kinds of variability in phenotypic traits and genomic content. The strains exhibited phase variation in phenotypes including pathogenicity and soft rot on potato. We showed that the presence of two configurations of the Type III Secretion System [single (S-PAI) and tripartite (T-PAI) pathogenicity islands] are not correlated with pathogenicity or with the capacity to induce soft rot in contrast to previous reports. The presence/absence of the avrE effector gene was the only trait we found to be correlated with pathogenicity of P.viridiflava. Other Type III secretion effector genes were not correlated with pathogenicity. A genomic region resembling an exchangeable effector locus (EEL) was found in S-PAI strains, and a probable recombination between the two PAIs is described. The ensemble of the variability observed in these phylogroups of P.syringae likely contributes to their adaptability to alternating opportunities for pathogenicity or saprophytic survival.

Original languageEnglish (US)
Pages (from-to)2301-2315
Number of pages15
JournalEnvironmental Microbiology
Volume16
Issue number7
DOIs
StatePublished - 2014

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Pseudomonas viridiflava
pathogenicity
phenotypic plasticity
species complex
Pseudomonas
Virulence
genetic variation
Ecosystem
secretion
genomics
pathogenicity islands
Pseudomonas syringae
Genomic Islands
type III secretion system
aquatic habitat
Solanum tuberosum
Agriculture
gene
Genetic Recombination
Genes

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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The Pseudomonas viridiflava phylogroups in the P.syringae species complex are characterized by genetic variability and phenotypic plasticity of pathogenicity-related traits. / Bartoli, Claudia; Berge, Odile; Monteil, Caroline L.; Guilbaud, Caroline; Balestra, Giorgio M.; Varvaro, Leonardo; Jones, Corbin; Dangl, Jeffery L.; Baltrus, David A; Sands, David C.; Morris, Cindy E.

In: Environmental Microbiology, Vol. 16, No. 7, 2014, p. 2301-2315.

Research output: Contribution to journalArticle

Bartoli, Claudia ; Berge, Odile ; Monteil, Caroline L. ; Guilbaud, Caroline ; Balestra, Giorgio M. ; Varvaro, Leonardo ; Jones, Corbin ; Dangl, Jeffery L. ; Baltrus, David A ; Sands, David C. ; Morris, Cindy E. / The Pseudomonas viridiflava phylogroups in the P.syringae species complex are characterized by genetic variability and phenotypic plasticity of pathogenicity-related traits. In: Environmental Microbiology. 2014 ; Vol. 16, No. 7. pp. 2301-2315.
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