The putative AKH receptor of the tobacco hornworm, Manduca sexta, and its expression

R. Ziegler, J. Isoe, Wendy Moore Brusca, Michael A Riehle, M. A. Wells

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Adipokinetic hormones are peptide hormones that mobilize lipids and/or carbohydrates for flight in adult insects and activate glycogen Phosphorylase in larvae during starvation and during molt. We previously examined the functional roles of adipokinetic hormone in Manduca sexta L. (Lepidoptera: Sphingidae). Here we report the cloning of the full-length cDNA encoding the putative adipokinetic hormone receptor from the fat body of M. sexta. The sequence analysis shows that the deduced amino acid sequence shares common motifs of G protein-coupled receptors, by having seven hydrophobic transmembrane segments. We examined the mRNA expression pattern of the adipokinetic hormone receptor by quantitative Real-Time PCR in fat body during development and in different tissues and found the strongest expression in fat body of larvae two days after molt to the fifth instar. We discuss these results in relation to some of our earlier results. We also compare the M. sexta adipokinetic hormone receptor with the known adipokinetic hormone receptors of other insects and with gonadotropin releasing hormone-like receptors of invertebrates.

Original languageEnglish (US)
Article number40
JournalJournal of Insect Science
Volume11
DOIs
StatePublished - 2011

Fingerprint

Manduca
Manduca sexta
fat body
adipokinetic hormone
receptors
Fat Body
molting
Sphingidae
peptide hormones
Larva
Insects
phosphorylase
larvae
imagos
gonadotropin-releasing hormone
glycogen
LHRH Receptors
starvation
Glycogen Phosphorylase
molecular cloning

Keywords

  • Adipokinetic hormone
  • adipokinetic hormone receptor
  • fat body
  • mRNA expression
  • Real Time PCR

ASJC Scopus subject areas

  • Insect Science
  • Medicine(all)

Cite this

The putative AKH receptor of the tobacco hornworm, Manduca sexta, and its expression. / Ziegler, R.; Isoe, J.; Moore Brusca, Wendy; Riehle, Michael A; Wells, M. A.

In: Journal of Insect Science, Vol. 11, 40, 2011.

Research output: Contribution to journalArticle

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