The rapid reduction of disulfiram in blood and plasma

J. Cobby, Michael Mayersohn, S. Selliah

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

A gas chromatographic assay procedure was developed to quantitate the reduction product of disulfiram, diethyldithiocarbamate (DDC), in blood and plasma. The procedure involved the in situ methylation of DDC prior to the extraction and chromatography of the methyl ester. The minimal sensitivity achieved was 0.2 μg/ml from 1 ml of blood or plasma. The coefficient of variation about any concentration was 10.5%. Calibration curves having a reproducible nonlinear form were prepared up to 9 μg/ml. The assay procedure was used to evaluate the stability of disulfiram and DDC in blood. Disulfiram was rapidly and quantitatively reduced to DDC within 4 minutes. The DDC thus formed decomposed in human and dog blood with half lives of 70 and 100 minutes respectively. The implications of these findings are discussed with respect to the chemical form of disulfiram responsible for the ethanol-sensitizing effect.

Original languageEnglish (US)
Pages (from-to)724-731)
JournalJournal of Pharmacology and Experimental Therapeutics
Volume202
Issue number3
StatePublished - 1977
Externally publishedYes

Fingerprint

Disulfiram
Ditiocarb
Methylation
Calibration
Chromatography
Esters
Ethanol
Gases
Dogs

ASJC Scopus subject areas

  • Pharmacology

Cite this

The rapid reduction of disulfiram in blood and plasma. / Cobby, J.; Mayersohn, Michael; Selliah, S.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 202, No. 3, 1977, p. 724-731).

Research output: Contribution to journalArticle

@article{a1fe30d931d14cfdbd80dd6864c70ffc,
title = "The rapid reduction of disulfiram in blood and plasma",
abstract = "A gas chromatographic assay procedure was developed to quantitate the reduction product of disulfiram, diethyldithiocarbamate (DDC), in blood and plasma. The procedure involved the in situ methylation of DDC prior to the extraction and chromatography of the methyl ester. The minimal sensitivity achieved was 0.2 μg/ml from 1 ml of blood or plasma. The coefficient of variation about any concentration was 10.5{\%}. Calibration curves having a reproducible nonlinear form were prepared up to 9 μg/ml. The assay procedure was used to evaluate the stability of disulfiram and DDC in blood. Disulfiram was rapidly and quantitatively reduced to DDC within 4 minutes. The DDC thus formed decomposed in human and dog blood with half lives of 70 and 100 minutes respectively. The implications of these findings are discussed with respect to the chemical form of disulfiram responsible for the ethanol-sensitizing effect.",
author = "J. Cobby and Michael Mayersohn and S. Selliah",
year = "1977",
language = "English (US)",
volume = "202",
pages = "724--731)",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "3",

}

TY - JOUR

T1 - The rapid reduction of disulfiram in blood and plasma

AU - Cobby, J.

AU - Mayersohn, Michael

AU - Selliah, S.

PY - 1977

Y1 - 1977

N2 - A gas chromatographic assay procedure was developed to quantitate the reduction product of disulfiram, diethyldithiocarbamate (DDC), in blood and plasma. The procedure involved the in situ methylation of DDC prior to the extraction and chromatography of the methyl ester. The minimal sensitivity achieved was 0.2 μg/ml from 1 ml of blood or plasma. The coefficient of variation about any concentration was 10.5%. Calibration curves having a reproducible nonlinear form were prepared up to 9 μg/ml. The assay procedure was used to evaluate the stability of disulfiram and DDC in blood. Disulfiram was rapidly and quantitatively reduced to DDC within 4 minutes. The DDC thus formed decomposed in human and dog blood with half lives of 70 and 100 minutes respectively. The implications of these findings are discussed with respect to the chemical form of disulfiram responsible for the ethanol-sensitizing effect.

AB - A gas chromatographic assay procedure was developed to quantitate the reduction product of disulfiram, diethyldithiocarbamate (DDC), in blood and plasma. The procedure involved the in situ methylation of DDC prior to the extraction and chromatography of the methyl ester. The minimal sensitivity achieved was 0.2 μg/ml from 1 ml of blood or plasma. The coefficient of variation about any concentration was 10.5%. Calibration curves having a reproducible nonlinear form were prepared up to 9 μg/ml. The assay procedure was used to evaluate the stability of disulfiram and DDC in blood. Disulfiram was rapidly and quantitatively reduced to DDC within 4 minutes. The DDC thus formed decomposed in human and dog blood with half lives of 70 and 100 minutes respectively. The implications of these findings are discussed with respect to the chemical form of disulfiram responsible for the ethanol-sensitizing effect.

UR - http://www.scopus.com/inward/record.url?scp=0012081674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012081674&partnerID=8YFLogxK

M3 - Article

C2 - 197231

AN - SCOPUS:0012081674

VL - 202

SP - 724-731)

JO - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 3

ER -