The relationship of interaction forces in the protein adsorption onto polymeric microspheres

Jeong-Yeol Yoon, Jung Hyun Kim, Woo Sik Kim

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The relationship between hydrophobic and electrostatic interactions in protein adsorption was studied with various sulfonated microspheres, and it was compared with carboxylated microspheres. Hydrophobic interaction governed the adsorption in the low sulfonated microspheres and electrostatic interaction did in the high sulfonated ones. The transition point was observed when the two forces were exactly balanced, but it was shifted to the left compared to the case of the carboxylated microspheres. The above adsorption experiments with different kinds of surface functionality revealed the following general mechanism of protein adsorption. The protein adsorption mainly occurs by hydrophobic interaction when the hydrophobic surface is slightly modified with weak or strong acid, while it primarily occurs by hydrogen bonding (or electrostatic) interaction when the surface is mostly modified with weak (or strong) acid. The adsorption by electrostatic interaction is higher than that by any other interactions, but the rate of adsorption is slowest. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume153
Issue number1-3
DOIs
StatePublished - Aug 15 1999
Externally publishedYes

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Microspheres
proteins
Proteins
Adsorption
adsorption
Coulomb interactions
electrostatics
interactions
acids
Acids
transition points
Hydrogen bonds
hydrogen
Experiments

Keywords

  • Bovine serum albumin
  • Electrostatic interaction
  • Hydrogen bonding
  • Hydrophobic interaction
  • Protein adsorption
  • Sulfonated microspheres

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

The relationship of interaction forces in the protein adsorption onto polymeric microspheres. / Yoon, Jeong-Yeol; Kim, Jung Hyun; Kim, Woo Sik.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 153, No. 1-3, 15.08.1999, p. 413-419.

Research output: Contribution to journalArticle

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