The release of endogenous amino acids into the vitreous of the intact eye of the albino rat: effect of light, potassium, and ouabain

B. M. Coull, D. K. Owens, R. W.P. Cutler

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The vitreal space of the intact eye of albino rats was perfused in vivo. The concentration of several endogenous amino acids in the vitreal effluent was measured by the [3H]microdansylation procedure. GABA was never detected despite a sensitivity of the method of 0.5 pmol. In contrast to previous results obtained in pigmented rats, photic stimulation with flashing white light did not alter the release of glycine or any of the other amino acids. Potassium (60 mM) and ouabain (0.1 mM) evoked a specific release of glycine. The potassium-evoked release was blocked by magnesium suggesting a neuronal site of origin of glycine. Ouabain-evoked release was not blocked by magnesium. The results were contrasted with experiments on radiolabeled amino acid release from retinas preloaded and superfused in vitro, a condition in which glial localization of exogenous amino acids predominates.

Original languageEnglish (US)
Pages (from-to)301-309
Number of pages9
JournalBrain Research
Volume210
Issue number1-2
DOIs
StatePublished - Apr 6 1981
Externally publishedYes

Keywords

  • amino acid
  • eye
  • light-evoked release
  • ouabain
  • potassium
  • vitreous

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'The release of endogenous amino acids into the vitreous of the intact eye of the albino rat: effect of light, potassium, and ouabain'. Together they form a unique fingerprint.

  • Cite this