The renal sodium-phosphate transporter and X-linked hypophosphatemic vitamin D-resistant rickets

James F. Collins, Fayez K Ghishan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The sodium-phosphate (Na+/P(i)) transporter is a brush-border membrane specific protein located in the epithelia of the kidney and intestine of mammals. This transporter is involved in the pathogenesis of X-linked hypophosphatemic rickets. X-linked hypophosphatemic vitamin D-resistant rickets is the most common form of rickets in man, and is characterized by low serum phosphate levels. The (Hyp) mouse is thought to be an animal homologue of this disease in humans. Decreased renal proximal tubular reabsorption of phosphate has been observed in the (Hyp) mouse, resulting in hyperphosphaturia. The Na+/P(i) transporter was cloned from the mouse, and cRNA generated from this cDNA showed increased sodium-dependent phosphate uptake in Xenopus oocytes. Northern blot analysis showed recognition of three transcripts in normal and (Hyp) mice at 2.6, 4.6, and 10 kilobases, with all three bands being decreased in the (Hyp) mouse. In-situ hybridization localized the message to the renal proximal tubules in both mice. Polyclonal antiserum, raised against a C-terminal synthetic peptide specific for this transporter, recognized several bands at 152, 87, 82, 77, and 37 kDa on Western blots of renal brush-border membrane proteins from both mice. The intensity of all bands was decreased in the (Hyp) mouse samples by 5-10 fold. Immunohistochemistry localized the immunoreactive protein to the apical membrane of the proximal tubules in both mice. The transcription rate of this gene was decreased by 2-fold in the (Hyp) mouse. Dietary phosphate deprivation resulted in increased transporter activity, message levels, and immunoreactive protein levels in both (Hyp) and control mice. Cloning of the PEX gene (from the Hyp) locus on chromosome X of patients with hypophosphatemic rickets would suggest that a product of the PEX gene alters the function of the Na+/P(i) transporter.

Original languageEnglish (US)
Pages (from-to)881-898
Number of pages18
JournalNutrition Research
Volume16
Issue number5
DOIs
StatePublished - May 1996

Fingerprint

Sodium-Phosphate Cotransporter Proteins
Hypophosphatemic Rickets
Kidney
Phosphates
Microvilli
Membrane Proteins
Familial Hypophosphatemic Rickets
Familial Hypophosphatemia
Genes
Complementary RNA
Proximal Kidney Tubule
Rickets
Animal Diseases
X Chromosome
Xenopus
Northern Blotting
Oocytes
Intestines
In Situ Hybridization

Keywords

  • Hyp mouse
  • Hypophosphatemic rickets
  • Kidney
  • Sodium-phosphate transporter

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The renal sodium-phosphate transporter and X-linked hypophosphatemic vitamin D-resistant rickets. / Collins, James F.; Ghishan, Fayez K.

In: Nutrition Research, Vol. 16, No. 5, 05.1996, p. 881-898.

Research output: Contribution to journalArticle

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