The role of cell bioaugmentation and gene bioaugmentation in the remediation of co-contaminated soils

Ian L. Pepper, Terry J. Gentry, Deborah T. Newby, Timberley M. Roane, Karen L. Josephson

Research output: Contribution to journalReview article

52 Scopus citations

Abstract

Soils co-contaminated with metals and organics present special problems for remediation. Metal contamination can delay or inhibit microbial of organic pollutants such that for effective in situ biodegradation, bioaugmentation is necessary. We monitored the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) or 3-chlorobenzoate (3-CB) in two different soils with and without cadmium (Cd) contamination. Additionally, we evaluated the ability of bioaugmentation to enhance organic degradation in these co-contaminated soils. Finally, we determined whether enhanced degradation was due to survival of the introduced organism (cell bioaugmentation) or plasmid transfer to indigenous microbial populations (gene bioaugmentation). In Brazito soil, dual inoculation with a Cd-resistant bacterium plus a known 2,4-D-degrading bacterium, Ralstonia eutropha JM134, enhanced 2,4-D-degradation. Escherichia coli D11, which lacks chromosomal genes necessary for complete 2,4-D mineralization, was used for gene bioaugmentation in Madera soil. Significant gene transfer of the plasmid to the indigenous populations was observed, and the rate of 2,4-D degradation was enhanced relative to that of controls. Cell bioaugmentation mentation was further demonstrated when Comamonas testosteroni was used to enhance biodegradation of 3-CB in Madera soil. In this case no transfer of plasmid pBRC60 to indigenous soil recipients was observed. For the Madera soil, nonbioaugmented samples ultimately showed complete 2,4-D degradation. In contrast, nonbioaugmented Brazito soils showed incomplete 2,4-D degradation. These studies are unique in showing that both cell bioaugmentation and gene bioaugmentation can be effective in enhancing organic degradation in co-contaminated soils. Ultimately, the bioaugmentation strategy may depend on the degree of contamination and the time frame available for remediation.

Original languageEnglish (US)
Pages (from-to)943-946
Number of pages4
JournalEnvironmental health perspectives
Volume110
Issue numberSUPPL. 6
DOIs
StatePublished - Dec 1 2002

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • Bioaugmentation
  • Bioremediation
  • Co-contaminated soil
  • Gene transfer
  • Heavy metals

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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