The role of endothelial nitric oxide synthase in the pathogenesis of a rat model of hepatopulmonary syndrome

M. B. Fallon, G. A. Abrams, B. Luo, Z. Hou, J. Dai, D. D. Ku

Research output: Contribution to journalArticle

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Abstract

Background and Aims: The hepatopulmonary syndrome occurs when intrapulmonary vasodilatation causes impaired arterial gas exchange in liver disease. The pathogenesis is poorly understood, although nitric oxide may be involved. Common bile duct ligation in the rat is a model of the hepatopulmonary syndrome, but no studies have evaluated NO in pulmonary vasodilatation in this model. The aim of this study was to determine whether NO contributes to intrapulmonary vasodilatation after bile duct ligation. Methods: Endothelial and inducible NO synthase (NOS) levels and localization and NO activity in pulmonary artery rings were assessed after bile duct ligation. Results: Pulmonary endothelial NOS levels increased and alveolar vascular staining was enhanced after bile duct ligation. No change in pulmonary inducible NOS levels or localization was detected. Increased endothelial NOS levels correlated with alterations in gas exchange and were accompanied by enhanced NO activity and a blunted response to phenylephrine, reversible by NOS inhibition, in pulmonary artery rings. Portal-vein-ligated animals, which do not develop intrapulmonary vasodilatation, had no changes in pulmonary NOS production or in NO activity in pulmonary artery rings. Conclusions: NO, derived from pulmonary vascular endothelial NOS, contributes to intrapulmonary vasodilatation in animal hepatopulmonary syndrome.

Original languageEnglish (US)
Pages (from-to)606-614
Number of pages9
JournalGastroenterology
Volume113
Issue number2
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Hepatopulmonary Syndrome
Nitric Oxide Synthase Type III
Nitric Oxide Synthase
Vasodilation
Ligation
Lung
Bile Ducts
Pulmonary Artery
Blood Vessels
Gases
Common Bile Duct
Phenylephrine
Portal Vein
Liver Diseases
Nitric Oxide
Staining and Labeling

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

The role of endothelial nitric oxide synthase in the pathogenesis of a rat model of hepatopulmonary syndrome. / Fallon, M. B.; Abrams, G. A.; Luo, B.; Hou, Z.; Dai, J.; Ku, D. D.

In: Gastroenterology, Vol. 113, No. 2, 01.01.1997, p. 606-614.

Research output: Contribution to journalArticle

Fallon, M. B. ; Abrams, G. A. ; Luo, B. ; Hou, Z. ; Dai, J. ; Ku, D. D. / The role of endothelial nitric oxide synthase in the pathogenesis of a rat model of hepatopulmonary syndrome. In: Gastroenterology. 1997 ; Vol. 113, No. 2. pp. 606-614.
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