The role of immunohistochemistry, electron microscopy, and ultrastructural cytochemistry in the diagnosis of mixed carcinoma-neuroendocrine neoplasms

Anna R. Graham, Claire M. Payee, Ray B. Nagle, Ellinor Angel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We studied four mixed carcinoma-neuroendocrine neoplasms from gastrointestinal tractand pancreas by routine light microscopy (LM), immunohistochemistry (zH), electron microscopy (EM), and ultrastructural cytochemistry (UC). By LM, the individual tumors showed fairly pure neuroendocrine (carcinoid) or epithelial (papillary) patterns, mixed neuroendocrine-carcinoma features and poorly-differentiated tumor in sheets and nests which did not lend itself to morphologic characterization. IH demonstrated mixed expression, within different areas of the same neoplasm, of epithelial antigens (keratins and carcinoembryonic antigen [CEA]) and neuroendocrine markers (neuron-specific enolase [NSE], bombesin and neurobormonal peptides). By EM, each tumor showed ultrastructural features of epithelial and neuroendocrine differentiation which varied substantially in terms of number of cells involved and their distribution; two of the neoplasms showed biphasic differentiation within single cells. The nature of the neurosecretory granules was verified with the uranaffin reaction (UR). This study illustrates the value of combining LM, IH, EM and UC for the identification of mixed carcinoma-neuroendocrine lesions.

Original languageEnglish (US)
Pages (from-to)23-33
Number of pages11
JournalPathology Research and Practice
Volume182
Issue number1
DOIs
StatePublished - Jan 1 1987

Keywords

  • Carcinoid
  • Gastric tract tumors
  • Mixed carcinoma-Neuroendocrine neoplasms
  • Pancreas tumors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

Fingerprint Dive into the research topics of 'The role of immunohistochemistry, electron microscopy, and ultrastructural cytochemistry in the diagnosis of mixed carcinoma-neuroendocrine neoplasms'. Together they form a unique fingerprint.

  • Cite this