The role of LORELEI in pollen tube reception at the interface of the synergid cell and pollen tube requires the modified eight-cysteine motif and the receptor-like kinase FERONIA

Xunliang Liu, Claudia Castro, Yanbing Wang, Jennifer Noble, Nathaniel Ponvert, Mark Bundy, Chelsea Hoel, Elena Shpak, Ravishankar Palanivelu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In angiosperms, pollen tube reception by the female gametophyte is required for sperm release and double fertilization. In Arabidopsis thaliana lorelei (lre) mutants, pollen tube reception fails in most female gametophytes, which thus remain unfertilized. LRE encodes a putative glycosylphosphatidylinositol (GPI)-anchored surface protein with a modified eight-cysteine motif (M8CM). LRE fused to citrine yellow fluorescent protein (LRE-cYFP) remains functional and localizes to the synergid plasma membrane-rich filiform apparatus, the first point of contact between the pollen tube and the female gametophyte. Structure-function analysis using LRE-cYFP showed that the role of LRE in pollen tube reception requires the M8CM, but not the domains required for GPI anchor addition. Consistently, LRE-cYFP-TM, where GPI anchor addition domains were replaced with a single-pass transmembrane domain, fully complemented the pollen tube reception defect in lre-7 female gametophytes. Ectopically expressed and delivered LRE-cYFP from pollen tubes could non-cell-autonomously complement the pollen tube reception defect in lre female gametophytes, only if they expressed FERONIA. Additionally, pollen tube-expressing LRE variants lacking domains critical for GPI anchor addition also rescued lre female gametophyte function. Therefore, LRE and FERONIA jointly function in pollen tube reception at the interface of the synergid cell and pollen tube.

Original languageEnglish (US)
Pages (from-to)1035-1052
Number of pages18
JournalPlant Cell
Volume28
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

Pollen Tube
pollen tubes
Cysteine
cysteine
phosphotransferases (kinases)
Phosphotransferases
receptors
Glycosylphosphatidylinositols
Ovule
cells
gametophytes
Proteins
Angiosperms
surface proteins
Arabidopsis
Fertilization
Spermatozoa
Angiospermae
Membrane Proteins
complement

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

The role of LORELEI in pollen tube reception at the interface of the synergid cell and pollen tube requires the modified eight-cysteine motif and the receptor-like kinase FERONIA. / Liu, Xunliang; Castro, Claudia; Wang, Yanbing; Noble, Jennifer; Ponvert, Nathaniel; Bundy, Mark; Hoel, Chelsea; Shpak, Elena; Palanivelu, Ravishankar.

In: Plant Cell, Vol. 28, No. 5, 01.05.2016, p. 1035-1052.

Research output: Contribution to journalArticle

Liu, Xunliang ; Castro, Claudia ; Wang, Yanbing ; Noble, Jennifer ; Ponvert, Nathaniel ; Bundy, Mark ; Hoel, Chelsea ; Shpak, Elena ; Palanivelu, Ravishankar. / The role of LORELEI in pollen tube reception at the interface of the synergid cell and pollen tube requires the modified eight-cysteine motif and the receptor-like kinase FERONIA. In: Plant Cell. 2016 ; Vol. 28, No. 5. pp. 1035-1052.
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