The role of the G protein γ2 subunit in opioid antinociception in mice

Keiko Hosohata, Jennifer K. Logan, Eva Varga, Thomas H. Burkey, Todd W. Vanderah, Frank Porreca, Victor J. Hruby, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.

Original languageEnglish (US)
Pages (from-to)R9-R11
JournalEuropean Journal of Pharmacology
Volume392
Issue number3
DOIs
StatePublished - Mar 31 2000

Keywords

  • Antinociception
  • DPDPE (c[D-Pen, D-Pen]enkephalin)
  • G protein γ subunit

ASJC Scopus subject areas

  • Pharmacology

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