The role of the G protein γ2 subunit in opioid antinociception in mice

Keiko Hosohata, Jennifer K. Logan, Eva Varga, Thomas H. Burkey, Todd W Vanderah, Frank Porreca, Victor J Hruby, William R Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2,D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE. Copyright (C) 2000.

Original languageEnglish (US)
JournalEuropean Journal of Pharmacology
Volume392
Issue number3
DOIs
StatePublished - Mar 31 2000

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D-Penicillamine (2,5)-Enkephalin
Oligodeoxyribonucleotides
Protein Subunits
GTP-Binding Proteins
Opioid Analgesics
Analgesia

Keywords

  • Antinociception
  • DPDPE (c[D-Pen,D-Pen]enkephalin)
  • G protein γ subunit

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

The role of the G protein γ2 subunit in opioid antinociception in mice. / Hosohata, Keiko; Logan, Jennifer K.; Varga, Eva; Burkey, Thomas H.; Vanderah, Todd W; Porreca, Frank; Hruby, Victor J; Roeske, William R; Yamamura, Henry I.

In: European Journal of Pharmacology, Vol. 392, No. 3, 31.03.2000.

Research output: Contribution to journalArticle

Hosohata, Keiko ; Logan, Jennifer K. ; Varga, Eva ; Burkey, Thomas H. ; Vanderah, Todd W ; Porreca, Frank ; Hruby, Victor J ; Roeske, William R ; Yamamura, Henry I. / The role of the G protein γ2 subunit in opioid antinociception in mice. In: European Journal of Pharmacology. 2000 ; Vol. 392, No. 3.
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