The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells

Melania E. Mercado-Pimentel, Benjamin C. Onyeagucha, Qing Li, Angel C. Pimentel, Jana Jandova, Mark A. Nelson

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Abstract S100P signaling through the receptor for advanced glycation end-products (RAGE) contributes to colon cancer invasion and metastasis, but the mechanistic features of this process are obscure. Here, we investigate whether activation of S100P/RAGE signaling regulates oncogenic microRNA-21 (miR-21). We show that exogenous S100P up-regulates miR-21 levels in human colon cancer cells, whereas knockdown of S100P results in a decrease of miR-21. Furthermore, blockage of RAGE with anti-RAGE antibody suppresses S100P induction of miR-21. In addition, we found that S100P induction of miR-21 expression involves ERK and is suppressed by the MEK inhibitor U0126. Also, S100P treatment stimulates the enrichment of c-Fos, and AP-1 family members, at the miR-21 gene promoter.

Original languageEnglish (US)
Article number37267
Pages (from-to)2388-2393
Number of pages6
JournalFEBS Letters
Volume589
Issue number18
DOIs
StatePublished - Aug 12 2015

Keywords

  • AP-1
  • Colon cancer
  • Inflammation
  • Metastasis
  • RAGE
  • RECK
  • TCGA
  • miR-21
  • microRNAs

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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